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422d
From Proteopedia
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5'-R(*GP*AP*UP*CP*AP*CP*UP*UP*CP*GP*GP*U)-3'
Overview
The crystal structure of an RNA dodecamer, r(GAUCACUUCGGU), was solved at 2.6 A resolution by the molecular-replacement method and refined to an R(work) of 18.8% (R(free) = 22.8%) using 2494 reflections. The dodecamer crystallized in the monoclinic space group C2, with unit-cell parameters a = 71.34, b = 39.98, c = 32.47 A, beta = 104.7 degrees and two independent strands in the asymmetric unit. The dodecamer adopts an octamer duplex structure with four 5'-overhang residues (G1A2U3C4), which form Watson-Crick base pairs with another four 5'-overhang residues of a symmetry-related duplex. The octamer duplex (ACUUCGGU) contains at its center four mismatched base pairs flanked by two Watson-Crick base pairs. The mismatched bases form two G.U wobble base pairs at the ends and two U.C base pairs at the center, with one base-base hydrogen bond N4(C).O4(U) and a water bridge connecting the N(3) of the cytosine and uridine. The present study reinforces the concept of the stability of the conformation of UUCG in RNA double-helical structures.
About this Structure
422D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structure of the dodecamer r(GAUCACUUCGGU) with four 5'-overhang nucleotides., Eswaramoorthy S, Rao ST, Pan B, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):8-12. Epub 2003 Dec, 18. PMID:14684886
Page seeded by OCA on Thu Feb 21 19:12:03 2008
