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spinqualitylabelsall models 1dxp, resolution 2.4Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

INHIBITION OF THE HEPATITIS C VIRUS NS3/4A PROTEASE. THE CRYSTAL STRUCTURES OF TWO PROTEASE-INHIBITOR COMPLEXES (APO STRUCTURE)

Overview

The hepatitis C virus NS3 protein contains a serine protease domain with a, chymotrypsin-like fold, which is a target for development of therapeutics., We report the crystal structures of this domain complexed with NS4A, cofactor and with two potent, reversible covalent inhibitors spanning the, P1-P4 residues. Both inhibitors bind in an extended backbone conformation, forming an anti-parallel beta-sheet with one enzyme beta-strand. The P1, residue contributes most to the binding energy, whereas P2-P4 side chains, are partially solvent exposed. The structures do not show notable, rearrangements of the active site upon inhibitor binding. These results, are significant for the development of antivirals.

About this Structure

1DXP is a [Protein complex] structure of sequences from [Gb virus c] and [Hepatitis c virus genotype 1a (isolate 1)] with ZN and GOL as [ligands]. Structure known Active Sites: ZN1 and ZN2. Full crystallographic information is available from [OCA].

Reference

Inhibition of the hepatitis C virus NS3/4A protease. The crystal structures of two protease-inhibitor complexes., Di Marco S, Rizzi M, Volpari C, Walsh MA, Narjes F, Colarusso S, De Francesco R, Matassa VG, Sollazzo M, J Biol Chem. 2000 Mar 10;275(10):7152-7. PMID:10702283

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