3b12

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Template:STRUCTURE 3b12

Contents

Crystal Structure of the Fluoroacetate Dehalogenase D104 mutant from Burkholderia sp. FA1 in complex with fluoroacetate

Template:ABSTRACT PUBMED 22674735

Function

[DEHA_BURSP] Catalyzes the hydrolytic defluorination of fluoroacetate to produce glycolate. Has only very low activity towards chloroacetate.[REFERENCE:1][1]

About this Structure

3b12 is a 2 chain structure with sequence from Burkholderia sp.. Full crystallographic information is available from OCA.

Reference

  • Nakayama T, Kamachi T, Jitsumori K, Omi R, Hirotsu K, Esaki N, Kurihara T, Yoshizawa K. Substrate Specificity of Fluoroacetate Dehalogenase: An Insight from Crystallographic Analysis, Fluorescence Spectroscopy, and Theoretical Computations. Chemistry. 2012 Jun 1. doi: 10.1002/chem.201103369. PMID:22674735 doi:10.1002/chem.201103369
  1. ↑ Jitsumori K, Omi R, Kurihara T, Kurata A, Mihara H, Miyahara I, Hirotsu K, Esaki N. X-Ray crystallographic and mutational studies of fluoroacetate dehalogenase from Burkholderia sp. strain FA1. J Bacteriol. 2009 Apr;191(8):2630-7. Epub 2009 Feb 13. PMID:19218394 doi:10.1128/JB.01654-08

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