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6fit
From Proteopedia
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FHIT-TRANSITION STATE ANALOG
Overview
The histidine triad (HIT) protein family is among the most ubiquitous and, highly conserved in nature, but a biological activity has not yet been, identified for any member of the HIT family. Fragile histidine triad, protein (FHIT) and protein kinase C interacting protein (PKCI) were used, in a structure-based approach to elucidate characteristics of in vivo, ligands and reactions. Crystallographic structures of apo, substrate, analog, pentacovalent transition-state analog, and product states of both, enzymes reveal a catalytic mechanism and define substrate characteristics, required for catalysis, thus unifying the HIT family as nucleotidyl, hydrolases, transferases, or both. The approach described here may be, useful in identifying structure-function relations between protein, families ... [(full description)]
About this Structure
6FIT is a [Single protein] structure of sequence from [Homo sapiens] with AMW as [ligand]. Active as [Bis(5'-adenosyl)-triphosphatase], with EC number [3.6.1.29]. Structure known Active Sites: AVE and HNE. Full crystallographic information is available from [OCA].
Reference
Structure-based analysis of catalysis and substrate definition in the HIT protein family., Lima CD, Klein MG, Hendrickson WA, Science. 1997 Oct 10;278(5336):286-90. PMID:9323207
Page seeded by OCA on Tue Oct 30 17:49:45 2007
Categories: Bis(5'-adenosyl)-triphosphatase | Homo sapiens | Single protein | Hendrickson, W.A. | Klein, M.G. | Lima, C.D. | AMW | Fhit | Fragile histidine triad protein | Histidine triad protein family | Hit protein family | Hydrolase | Nucleotidyl hydrolase | Nucleotidyl transferase | Putative tumor suppressor
