1qkr is a 2 chain structure with sequence from Gallus gallus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Vinculin plays a dynamic role in the assembly of the actin cytoskeleton. A strong interaction between its head and tail domains that regulates binding to other cytoskeletal components is disrupted by acidic phospholipids. Here, we present the crystal structure of the vinculin tail, residues 879-1066. Five amphipathic helices form an antiparallel bundle that resembles exchangeable apolipoproteins. A C-terminal arm wraps across the base of the bundle and emerges as a hydrophobic hairpin surrounded by a collar of basic residues, adjacent to the N terminus. We show that the C-terminal arm is required for binding to acidic phospholipids but not to actin, and that binding either ligand induces conformational changes that may represent the first step in activation.
Crystal structure of the vinculin tail suggests a pathway for activation.,Bakolitsa C, de Pereda JM, Bagshaw CR, Critchley DR, Liddington RC Cell. 1999 Dec 10;99(6):603-13. PMID:10612396[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
↑ Bakolitsa C, de Pereda JM, Bagshaw CR, Critchley DR, Liddington RC. Crystal structure of the vinculin tail suggests a pathway for activation. Cell. 1999 Dec 10;99(6):603-13. PMID:10612396
