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Life originated, according to the RNA World hypothesis, from self-replicating ribozymes that catalyzed ligation of RNA fragments. We have solved the 2.6 angstrom crystal structure of a ligase ribozyme that catalyzes regiospecific formation of a 5' to 3' phosphodiester bond between the 5'-triphosphate and the 3'-hydroxyl termini of two RNA fragments. Invariant residues form tertiary contacts that stabilize a flexible stem of the ribozyme at the ligation site, where an essential magnesium ion coordinates three phosphates. The structure of the active site permits us to suggest how transition-state stabilization and a general base may catalyze the ligation reaction required for prebiotic RNA assembly.
The structural basis of ribozyme-catalyzed RNA assembly.,Robertson MP, Scott WG Science. 2007 Mar 16;315(5818):1549-53. PMID:17363667[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
↑ Robertson MP, Scott WG. The structural basis of ribozyme-catalyzed RNA assembly. Science. 2007 Mar 16;315(5818):1549-53. PMID:17363667 doi:315/5818/1549
