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3p1y
From Proteopedia
Contents |
Crystal structure of the chimeric Archaeoglobus fulgidus RNA splicing endonuclease with the broadest substrate specificity
Template:ABSTRACT PUBMED 21846775
Function
[ENDA_ARCFU] Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
About this Structure
3p1y is a 4 chain structure with sequence from Aeropyrum pernix. Full crystallographic information is available from OCA.
Reference
- Hirata A, Kitajima T, Hori H. Cleavage of intron from the standard or non-standard position of the precursor tRNA by the splicing endonuclease of Aeropyrum pernix, a hyper-thermophilic Crenarchaeon, involves a novel RNA recognition site in the Crenarchaea specific loop. Nucleic Acids Res. 2011 Nov;39(21):9376-89. Epub 2011 Aug 16. PMID:21846775 doi:10.1093/nar/gkr615
