| Structural highlights
1sxj is a 8 chain structure with sequence from Saccharomyces cerevisiae. The June 2012 RCSB PDB Molecule of the Month feature on Sliding Clamps by David Goodsell is 10.2210/rcsb_pdb/mom_2012_6. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Ligands: | , ,
| | NonStd Res: | , |
| Related: | 1plq |
| Gene: | RFC1, CDC44, YOR217W, YOR50-7 (Saccharomyces cerevisiae), RFC4, YOL094C, O0923 (Saccharomyces cerevisiae), RFC3, YNL290W, N0533 (Saccharomyces cerevisiae), RFC2, YJR068W, J1808 (Saccharomyces cerevisiae), RFC5, YBR087W, YBR0810 (Saccharomyces cerevisiae), POL30, YBR088C, YBR0811 (Saccharomyces cerevisiae) |
| Resources: | FirstGlance, OCA, RCSB, PDBsum |
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Sliding clamps are ring-shaped proteins that encircle DNA and confer high processivity on DNA polymerases. Here we report the crystal structure of the five-protein clamp loader complex (replication factor-C, RFC) of the yeast Saccharomyces cerevisiae, bound to the sliding clamp (proliferating cell nuclear antigen, PCNA). Tight interfacial coordination of the ATP analogue ATP-gammaS by RFC results in a spiral arrangement of the ATPase domains of the clamp loader above the PCNA ring. Placement of a model for primed DNA within the central hole of PCNA reveals a striking correspondence between the RFC spiral and the grooves of the DNA double helix. This model, in which the clamp loader complex locks onto primed DNA in a screw-cap-like arrangement, provides a simple explanation for the process by which the engagement of primer-template junctions by the RFC:PCNA complex results in ATP hydrolysis and release of the sliding clamp on DNA.
Structural analysis of a eukaryotic sliding DNA clamp-clamp loader complex.,Bowman GD, O'Donnell M, Kuriyan J Nature. 2004 Jun 17;429(6993):724-30. PMID:15201901[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Bowman GD, O'Donnell M, Kuriyan J. Structural analysis of a eukaryotic sliding DNA clamp-clamp loader complex. Nature. 2004 Jun 17;429(6993):724-30. PMID:15201901 doi:10.1038/nature02585
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