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2z5l
From Proteopedia
Revision as of 05:14, 2 October 2014 by OCA (Talk | contribs)
2z5l is a 1 chain structure with sequence from Streptomyces fradiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Because it controls the majority of polyketide stereocenters, the ketoreductase (KR) is a central target in engineering polyketide synthases (PKSs). To elucidate the mechanisms of stereocontrol, the structure of KR from the first module of the tylosin PKS was determined. A comparison with a recently solved erythromycin KR that operates on the same substrate explains why their products have opposite alpha-substituent chiralities. The structure reveals how polyketides are guided into the active site by key residues in different KR types. There are four types of reductase-competent KRs, each capable of fixing a unique combination of alpha-substituent and beta-hydroxyl group chiralities, as well as two types of reductase-incompetent KRs that control alpha-substituent chirality alone. A protocol to assign how a module will enforce substituent chirality based on its sequence is presented.
A tylosin ketoreductase reveals how chirality is determined in polyketides.,Keatinge-Clay AT Chem Biol. 2007 Aug;14(8):898-908. PMID:17719489[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
↑ Keatinge-Clay AT. A tylosin ketoreductase reveals how chirality is determined in polyketides. Chem Biol. 2007 Aug;14(8):898-908. PMID:17719489 doi:10.1016/j.chembiol.2007.07.009
