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Template:STRUCTURE 2zfi

3D Structure of Kinesin
The image at the left is a Crystal Structure of the Kif1A Motor Domain Before Mg Release (2zfi)

Contents 1 Kinesin 2 3D Structures of Kinesin 2.1 Kinesin Heavy Chain (KIF) 2.2 Kinesin light chain (KLC) 2.3 Kinesin-14 2.4 Eg5 2.5 KCBP 2.6 NOD

Kinesin

Kinesins are eukaryotic motor proteins which move along microtubules. Kinesin (KIF) is a dimer consisting of 2 heavy chains and two light chains. The heavy chain contains the globular motor domain (MD), flexible neck linker (FNL) and a long coiled-coil stalk which intertwines to form the dimer. The light chain (KLC) forms the tail region. The KLC contains a cargo binding domain which is called TPR (Tetratricopeptide repeat). The KIFs are named by their gene number. KIF contains a forkhead-associated domain (FHA) which is involved in phosphopeptide recognition. Kinesin protein in yeast is called Kar3. Eg5 or KIF11 is a kinesin (Kinesin-5) which participates in mitosis. KCBP is a Kinesin-like Calmodulin Binding Protein. NOD is a Drosophila chromosome-associated kinesin.

3D Structures of Kinesin

Update November 2011

Kinesin Heavy Chain (KIF)

2wbe – cKIF MD+FNL+Tubulin A+Tubulin B+AMPPNP – cow
1ia0 – KIF1A MD+Tubulin A+Tubulin B+ATP - pig
3gbj – hKIF13B MD+ADP – human
3edl – cKIF MD+ Tubulin A+Tubulin B – EM
2zfi, 2zfj, 2zfk, 2zfl, 2zfm – mKIF1A – mouse
1vfv, 1vfw – mKIF1A MD+Mg-AMPPNP
1vfx - mKIF1A MD+ADP-Mg-AlFx
1vfz - mKIF1A MD+ADP-Mg-VO4
1v8j – mKIF2C MD+Mg-ADP
1i5s - mKIF2C MD (mutant)+Mg-ADP
1i6i - mKIF2C MD (mutant)+Mg-AMPPCP
1v8k - mKIF2C MD+Mg-AMPPNP
2p4n – hKIF+ Tubulin A+TubulinB – EM docking
2vvg – KIF2 MD – Giardia intestinalis
2cjo – hKIF13+inhibitor 30
2nr8 – hKIF9+ADP
2g1l - hKIF1C FHA domain
2owm – NcKIF1C MD – Neurospora crassa
1goj – NcKIF MD
3bfn – hKIF22 MD
3b6u, 3b6v – hKIF3B MD
2rep – hKIFC1 MD
2eh0 - hKIF1B FHA domain – NMR
2edu – hKIF22 C-terminal – NMR
2v14 – hKIF16B
2heh – hKIF2C
2h58 – hKIFC3+ADP
2gry – hKIF2+ADP
2cow – hKIF13B CAP-gly domain – NMR
1mkj – hKIF MD+docked FNL
1bg2 – hKIF MD
3kin, 2kin – KIF MD - rat
2hfx – pKIF1A head+AMPPNP – pig
2hxh - pKIF1A head+ADP
1ry6 – KIF MD – Plasmodium falciparum
2ncd, 1cz7 – DmKIF MD non-claret disjuctional (NCD) dimer – Drosophila melanogaster
1n6m – DmKIF MD+FNL
2y5w, 2y65 - DmKIF MD

Kinesin light chain (KLC)

3edt – hKLC2
3ceq, 3nf1 – hKLC2 TPR domain

Kinesin-14

1f9t, 1f9u, 1f9v, 1f9w, 3kar - Kar3 MD (mutant) - yeast
3l1c – DmNCD (mutant)

Eg5

3ken - hKIF11 MD+S-trityl-L-cysteine
2q2y, 2fky, 2q2z, 2g1q, 2fl2, 2fl6, 1yrs - hKIF11+inhibitors
2pg2 - hKIF11+inhibitor 15+ADP
2uyi - hKIF11+inhibitor 33+ADP
2uym - hKIF11+inhibitor 37+ADP
2ieh - hKIF11+inhibitor (R)-mon97
2fme, 3l9h - hKIF11+quinoline inhibitor
1x88, 1q0b - hKIF11 MD+MgADP+monastrol
3k3b - hKIF11 MD+tetrahydro-beta-carboline
3hqd - hKIF11 MD+AMPPNP+Mg
1ii6 - hKIF11 MD+ADP+Mg
3k5e - hKIF11 MD+ADP+enastrol

KCBP

3h4s – KCBP+ADP+Mg – Arabidopsis thaliana

NOD

3dc4 – DmNOD catalytic core domain+ADP
3dcb - DmNOD catalytic core domain+AMPPNP
3dco - DmNOD catalytic core domain+ cTubulin A+cTubulin B