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2bjv

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Revision as of 14:02, 20 March 2008 by OCA (Talk | contribs)
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Image:2bjv.gif


PDB ID 2bjv

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, resolution 1.70Å
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE OF PSPF(1-275) R168A MUTANT


Overview

Activators of bacterial sigma54-RNA polymerase holoenzyme are mechanochemical proteins that use adenosine triphosphate (ATP) hydrolysis to activate transcription. We have determined by cryogenic electron microscopy (cryo-EM) a 20 angstrom resolution structure of an activator, phage shock protein F [PspF(1-275)], which is bound to an ATP transition state analog in complex with its basal factor, sigma54. By fitting the crystal structure of PspF(1-275) at 1.75 angstroms into the EM map, we identified two loops involved in binding sigma54. Comparing enhancer-binding structures in different nucleotide states and mutational analysis led us to propose nucleotide-dependent conformational changes that free the loops for association with sigma54.

About this Structure

2BJV is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structural insights into the activity of enhancer-binding proteins., Rappas M, Schumacher J, Beuron F, Niwa H, Bordes P, Wigneshweraraj S, Keetch CA, Robinson CV, Buck M, Zhang X, Science. 2005 Mar 25;307(5717):1972-5. PMID:15790859

Page seeded by OCA on Thu Mar 20 16:02:54 2008

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