1ol0
From Proteopedia
|
CRYSTAL STRUCTURE OF A CAMELISED HUMAN VH
Overview
The variable domain of dromedary immunoglobulins comprises only the heavy, chain and is missing the light-chain variable domain. This single domain, is sufficient for antigen recognition and binding-half that required by, other mammals. Human antibody-VHs have previously been camelized to be, soluble stable fragments that retain antigen binding. Such engineered VHH, are of interest in drug development, since they are nonimmunogenic, and in, other biotechnology applications. We present the structure of a camelized, human antibody fragment (cVH), which is a competitive and reversible, inhibitor of the NS3 serine protease of the hepatitis C virus (HCV). In, solution, this cVH undergoes a concentration-dependent monomer-dimer, equilibrium. The structure confirms the minimum mutational requirements of, the VL-binding face. The fragment also suggests a means by which the, observed dimerization occurs, highlighting the importance of the, composition of the CDR3 in maintaining a truly camelized VH.
About this Structure
1OL0 is a Single protein structure of sequence from Homo sapiens with SO4 and GOL as ligands. Structure known Active Site: AC1. Full crystallographic information is available from OCA.
Reference
Crystal structure of a human VH: requirements for maintaining a monomeric fragment., Dottorini T, Vaughan CK, Walsh MA, LoSurdo P, Sollazzo M, Biochemistry. 2004 Jan 27;43(3):622-8. PMID:14730966
Page seeded by OCA on Mon Nov 5 13:08:22 2007
