2hvy
From Proteopedia
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| , resolution 2.30Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | truB (Pyrococcus furiosus), GAR1 (Pyrococcus furiosus), NOP10 (Pyrococcus furiosus), rpl7ae (Pyrococcus furiosus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of an H/ACA box RNP from Pyrococcus furiosus
Overview
H/ACA ribonucleoprotein particles (RNPs) are a family of RNA pseudouridine synthases that specify modification sites through guide RNAs. They also participate in eukaryotic ribosomal RNA processing and are a component of vertebrate telomerases. Here we report the crystal structure, at 2.3 A resolution, of an entire archaeal H/ACA RNP consisting of proteins Cbf5, Nop10, Gar1 and L7ae, and a single-hairpin H/ACA RNA, revealing a modular organization of the complex. The RNA upper stem is bound to a composite surface formed by L7ae, Nop10 and Cbf5, and the RNA lower stem and ACA signature motif are bound to the PUA domain of Cbf5, thereby positioning middle guide sequences so that they are primed to pair with substrate RNA. Furthermore, Gar1 may regulate substrate loading and release. The structure rationalizes the consensus structure of H/ACA RNAs, suggests a functional role of each protein, and provides a framework for understanding the mechanism of RNA-guided pseudouridylation, as well as various cellular functions of H/ACA RNP.
About this Structure
2HVY is a Protein complex structure of sequences from Pyrococcus furiosus. Full crystallographic information is available from OCA.
Reference
Crystal structure of an H/ACA box ribonucleoprotein particle., Li L, Ye K, Nature. 2006 Sep 21;443(7109):302-7. Epub 2006 Aug 30. PMID:16943774
Page seeded by OCA on Thu Mar 20 17:22:16 2008
Categories: Protein complex | Pyrococcus furiosus | Ye, K. | ATP | ZN | Guide rna | H/aca rna | Pseudouridine synthase | Rnp
