Transcription initiation factors (TFII) are proteins which enable RNA polymerase II to bind to the DNA template in order to start the transcription process.
- TFIIA is one of several TFs which are required for transcription. TFIIA aids in binding the TATA-binding protein (TBP) to the TATA box containing sequence of the DNA.
- TFIIB bridges between TFIID and RNA polymerase II.
- TFIID recognizes the promoter sequence on the DNA.
- TFIIF binds RNA polymerase III prohibiting it to bind to DNA outside the promoter.
3D structures of transcription initiation factors
Updated on 21-August-2014
TFIIA
1ytf, 1nh2, 1rm1 – yTFIIA + TBP + DNA – yeast
1nvp – hTFIIA + TATA-binding protein + DNA – human
TFIIB
1pft – TFIIB N terminal – Pyrococcus furiosus – NMR
1tfb – hTFIIB - NMR
1dl6 - hTFIIB N terminal – NMR
1rly - hTFIIB Zn ribbon domain + Zn – NMR
1ro4 - hTFIIB Zn ribbon domain – NMR
1vol, 1c9b – hTFIIB + TBP + DNA
3k1f, 3k7a, 4bbr, 4bbs – yTFIIB + RNA polymerase II
1ais, 1d3u - TFIIB C terminal + TBP + DNA – Pyrococcus woesei
TFIID
1bh8 – hTFIID
1eqf – hTFIID subunit 1
3uv5, 3uv4 – hTFIID subunit 1 bromodomain
3aad – hTFIID subunit 1 bromodomain + histone chaperone ASF1A
1tba – TFIID – Drosophila melanogaster - NMR
1bh9 – hTFIID + PCMBS
1qn3, 1qn4, 1qn5, 1qn6, 1qn7, 1qn8, 1qn9, 1qna, 1qnb, 1qnc, 1qne – TFIID + DNA – Arabidopsis thaliana
TFIIE
1vd4 - hTFIIB Zn finger domain – NMR
2rnq – hTFIIE C terminal - NMR
2rnr – hTFIIE C terminal + TFIIH PH domain- NMR
TFIIF
1f3u – hTFIIF
1nha – hTFIIF C terminal - NMR
1i27 – hTFIIF C terminal (mutant)
1j2x – hTFIIF C terminal + RNA polymerase CTD phosphatase peptide
1onv – hTFIIF C terminal + serine phosphatase peptide - NMR
2k7l – hTFIIF C terminal + FCP1 peptide - NMR
