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2v5o
From Proteopedia
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| , resolution 2.91Å | |||||||
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| Sites: | , , , and | ||||||
| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF HUMAN IGF2R DOMAINS 11-14
Overview
Embryonic development and normal growth require exquisite control of insulin-like growth factors (IGFs). In mammals the extracellular region of the cation-independent mannose-6-phosphate receptor has gained an IGF-II-binding function and is termed type II IGF receptor (IGF2R). IGF2R sequesters IGF-II; imbalances occur in cancers and IGF2R is implicated in tumour suppression. We report crystal structures of IGF2R domains 11-12, 11-12-13-14 and domains 11-12-13/IGF-II complex. A distinctive juxtaposition of these domains provides the IGF-II-binding unit, with domain 11 directly interacting with IGF-II and domain 13 modulating binding site flexibility. Our complex shows that Phe19 and Leu53 of IGF-II lock into a hydrophobic pocket unique to domain 11 of mammalian IGF2Rs. Mutagenesis analyses confirm this IGF-II 'binding-hotspot', revealing that IGF-binding proteins and IGF2R have converged on the same high-affinity site.
About this Structure
2V5O is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure and functional analysis of the IGF-II/IGF2R interaction., Brown J, Delaine C, Zaccheo OJ, Siebold C, Gilbert RJ, van Boxel G, Denley A, Wallace JC, Hassan AB, Forbes BE, Jones EY, EMBO J. 2008 Jan 9;27(1):265-76. Epub 2007 Nov 29. PMID:18046459
Page seeded by OCA on Thu Mar 20 18:43:25 2008
Categories: Homo sapiens | Single protein | Boxel, G Van. | Brown, J. | Delaine, C. | Denley, A. | Forbes, B E. | Gilbert, R J. | Hassan, A B. | Jones, E Y. | Siebold, C. | Wallace, J C. | Zaccheo, O J. | CL | NAG | Beta barrel | Cation independent mannose 6-phosphate | Fibronectin type ii | Glycoprotein | Insulin-like growth factor | Lysosome | Membrane | Phosphorylation | Polymorphism | Receptor | Transmembrane | Transport
