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4m5x
From Proteopedia
Revision as of 06:13, 24 September 2014 by OCA (Talk | contribs)
4m5x is a 2 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
A sparse-matrix screen for new crystallization conditions for the USP7 catalytic domain (USP7CD) led to the identification of a condition in which crystals grow reproducibly in 24-48 h. Variation of the halide metal, growth temperature and seed-stock concentration resulted in a shift in space group from P21 with two molecules in the asymmetric unit to C2 with one molecule in the asymmetric unit. Representative structures from each space group were determined to 2.2 A resolution and these structures support previous findings that the catalytic triad and switching loop are likely to be in unproductive conformations in the absence of ubiquitin (Ub). Importantly, the new structures reveal previously unobserved electron density for blocking loop 1 (BL1) residues 410-419. The new structures indicate a distinct rearrangement of the USP7 BL1 compared with its position in the presence of bound Ub.
A 2.2 A resolution structure of the USP7 catalytic domain in a new space group elaborates upon structural rearrangements resulting from ubiquitin binding.,Molland K, Zhou Q, Mesecar AD Acta Crystallogr F Struct Biol Commun. 2014 Mar;70(Pt 3):283-7. doi:, 10.1107/S2053230X14002519. Epub 2014 Feb 19. PMID:24598911[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
↑ Molland K, Zhou Q, Mesecar AD. A 2.2 A resolution structure of the USP7 catalytic domain in a new space group elaborates upon structural rearrangements resulting from ubiquitin binding. Acta Crystallogr F Struct Biol Commun. 2014 Mar;70(Pt 3):283-7. doi:, 10.1107/S2053230X14002519. Epub 2014 Feb 19. PMID:24598911 doi:http://dx.doi.org/10.1107/S2053230X14002519
