Structural highlights
3v11 is a 4 chain structure with sequence from Escherichia coli and Sulfolobus solfataricus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Ligands: | , , |
| NonStd Res: | , , , , |
| Related: | 2aho, 2qmu, 3cw5 |
| Gene: | eif2g, SSO0412 (Sulfolobus solfataricus), eif2a, SSO1050 (Sulfolobus solfataricus), eif2b, SSO2381 (Sulfolobus solfataricus) |
| Resources: | FirstGlance, OCA, RCSB, PDBsum |
Function
[IF2G_SULSO] eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (By similarity). [IF2B_SULSO] eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (By similarity). [IF2A_SULSO] eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (By similarity).
Publication Abstract from PubMed
Eukaryotic and archaeal translation initiation factor 2 (e/aIF2) is a heterotrimeric GTPase that has a crucial role in the selection of the correct start codon on messenger RNA. We report the 5-A resolution crystal structure of the ternary complex formed by archaeal aIF2 from Sulfolobus solfataricus, the GTP analog GDPNP and methionylated initiator tRNA. The 3D model is further supported by solution studies using small-angle X-ray scattering. The tRNA is bound by the alpha and gamma subunits of aIF2. Contacts involve the elbow of the tRNA and the minor groove of the acceptor stem, but not the T-stem minor groove. We conclude that despite considerable structural homology between the core gamma subunit of aIF2 and the elongation factor EF1A, these two G proteins of the translation apparatus use very different tRNA-binding strategies.
Structure of the ternary initiation complex aIF2-GDPNP-methionylated initiator tRNA.,Schmitt E, Panvert M, Lazennec-Schurdevin C, Coureux PD, Perez J, Thompson A, Mechulam Y Nat Struct Mol Biol. 2012 Mar 25;19(4):450-4. doi: 10.1038/nsmb.2259. PMID:22447243[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Schmitt E, Panvert M, Lazennec-Schurdevin C, Coureux PD, Perez J, Thompson A, Mechulam Y. Structure of the ternary initiation complex aIF2-GDPNP-methionylated initiator tRNA. Nat Struct Mol Biol. 2012 Mar 25;19(4):450-4. doi: 10.1038/nsmb.2259. PMID:22447243 doi:10.1038/nsmb.2259
