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Template:STRUCTURE 1rva Endonuclease (ENN) cleaves phosphodiester bond within polynucleotide chain. ENN cleaves DNA at a restriction site which is usually a 6-nucleotide palindrome. ENN is restriction site–specific. Various types of ENN differ by their mechanism of action. ENN is used in genetic engineering to make recombinant DNA. ENN requires a restriction site and a cleavage pattern. ENN-I operates on DNA with separate restriction site and cleavage pattern, while ENN-II operates on overlapping restriction site and cleavage pattern. Some ENNs are encoded within introns thus facilitating their mobility. These ENNs or inteins are designated I-ENN. See also

• Apurinic-Apyrimidinic Endonuclease-1
  
• DNA repair protein human endonuclease VIII-like 1 (NEIL1) .

Contents 1 3D structures of endonuclease 1.1 ENN-I 1.2 ENN-II 1.3 ENN-III 1.4 ENN-IV 1.5 ENN-V 1.6 ENN-VII 1.7 ENN-VIII 1.8 EcoKI ENN 1.9 Ecoo109I ENN 1.10 EcoRI ENN 1.11 EcoRII ENN 1.12 EcoRV ENN 1.13 BamHI ENN 1.14 FokI ENN 1.15 PvuII ENN 1.16 tRNA-splicing ENN 1.17 VSR ENN 1.18 BglI ENN 1.19 BglII ENN 1.20 MunI ENN 1.21 Sm ENN 1.22 NaeI ENN 1.23 NgoMIV ENN 1.24 NEI ENN 1.25 BsoBI ENN 1.26 Bse634I ENN 1.27 HincII ENN 1.28 HinDIII ENN 1.29 HinP1I ENN 1.30 Flap ENN 1.31 DNA mismatch ENN 1.32 Vvn ENN 1.33 BfiI ENN 1.34 SfiI ENN 1.35 Ecl18kI ENN 1.36 SdaI ENN 1.37 PabI ENN 1.38 PspGI ENN 1.39 NHN ENN 1.40 UV damage ENN 1.41 NotI ENN 1.42 BpujI ENN 1.43 Sau3AI ENN 1.44 HpyI ENN 1.45 PacI ENN 1.46 Crossover junction ENN 1.47 OkrAI ENN 1.48 BspD6I ENN 1.49 ThaI ENN 1.50 Xpf ENN 1.51 Intron-encoded endonuclease 1.52 I-PpoI ENN 1.53 I-CreI ENN 1.54 I-CeuI ENN 1.55 I-DmoI ENN 1.56 I-AniI ENN 1.57 I-HmuI ENN 1.58 I-TevI ENN 1.59 I-SceI ENN 1.60 I-PkoII ENN 1.61 I-MspI ENN 1.62 I-PfuI ENN 1.63 I-Tsp061I ENN 1.64 I-Vdi141I ENN 1.65 I-MtuI ENN 1.66 I-MsoI ENN 1.67 I-GzeMII ENN 1.68 I-tRNA ENN

3D structures of endonuclease

Updated on 17-August-2014

ENN-I

1bix - hENN – human
1cfr – ENN – Citrobacter freundii
1af5 - CrENN – Chlamydomonas reinhardtii
1fzr, 1m0d, 1m0i, 3cae – T7ENN - Enterobacteria phage T7
2g7e, 2g7f – VcENN – Vibrio cholera
2vnd - VcENN (mutant)
2pu3 - VsENN – Vibrio salmonicida

ENN-II

1pvi, 1pvu - ENN – Proteus vulgaris
2wsh - T4ENN (mutant) - Enterobacteria phage T4
3w52 – ENN – Arabidopsis thaliana

ENN-III

2abk, 1ako - EcENN – Escherichia coli
1orn, 1orp, 1p59 - GsENN + DNA – Geobacillus stearothermophilus

ENN-IV

1qtw - EcENN
2nqh - EcENN (mutant)
1qum – EcENN + DNA
2nqj, 2nq9, 4k1g - EcENN (mutant) + DNA
1xp3 - ENN – Bacillus anthracis
3aal - GkENN – Geobacillus kaustophilus
3aam - TtENN – Thermus thermophilus

ENN-V

1eni, 1enj, 1enk, 2end - T4ENN
1vas - T4ENN + DNA
2w35, 2w36, 4b20 - TmENN + DNA – Thermotoga maritima
3hd0 - TmENN
3ga2 - BsENN (mutant) – Bacillus subtilis
3goc - ENN – Streptomyces avermitilis
2fcc – T4ENN + DNA

ENN-VII

1en7, 1e7d - T4ENN
1e7l - T4ENN (mutant)
2qnc, 2qnf - T4ENN (mutant) + DNA

ENN-VIII

1q39, 1q39 - EcENN
1q3b, 1q3c - EcENN (mutant)
1tdh – hENN
1bix – hENN + DNA
1k3x, 2ea0, 1k3w, 1k3x, 2ea0, 2opf, 2oq4 - EcENN + DNA
2opf, 2oq4 - EcENN (mutant) + DNA

EcoKI ENN

2ar0 – EcENN (mutant)

Ecoo109I ENN

1wtd - EcENN
1wte – EcENN + DNA

EcoRI ENN

1qc9 - EcENN
1eri, 1ckq, 1cl8, 1qps – EcENN + DNA
1qrh, 1qri, 2oxv - EcENN (mutant) + DNA

EcoRII ENN

1na6 – EcENN
3hqf, 3hqg - EnENN + DNA

EcoRV ENN

1rve, 1az3 – EcENN
1az4 - EcENN (mutant)
2rve, 4rve, 1rva, 1rvb, 1rvc, 1az0, 1bsu, 1bua, 1bgb, 1rv5, 1b94, 1b95, 1eo3, 1eo4, 1eon, 1eoo, 1eop, 2b0d, 2b0e - EcENN + DNA
1bss, 1b96, 1b97, 1stx, 1suz, 1sx5, 1sx8, 2ge5 - EcENN (mutant) + DNA

BamHI ENN

1bam – BaENN – Bacillus amyloliquefaciens
1bhm, 3bam, 2bam, 1esg - BaENN + DNA

FokI ENN

1fok – PoENN + DNA – Planomicrobium okeanokoites
2fok – PoENN

PvuII ENN

3pvi - PvENN (mutant) + DNA – Proteus vulgaris
1k0z, 1h56, 3ksk - PvENN
1ni0 - PvENN (mutant)
2pvi, 1eyu, 1f0o - PvENN + DNA

tRNA-splicing ENN

1a79, 1a76, 1a77 - MjENN – Methanocaldococcus jannaschii
2cv8 - ENN – Sulfolobus tokodaii
2gw6 – hENN subunit sen15 – NMR
2gjw - AfENN + RNA – Archaeoglobus fulgidus
1r0v, 1r11 – AfENN 2ohc, 2ohe - TaENN – Thermoplasma acidophilum
2zyz – ENN – Pyrobaculum aerophilum
3iey - ENN + NEQ261 – Nanoarchaeum equitans
3ajv - ApENN (mutant) – Aeropyrum pernix
3p1y - ApENN /AfENN
3p1z - ApENN + protein

VSR ENN

1vsr – EcENN
1cw0 - EcENN + DNA

BglI ENN

1dmu, 1d2i – BsENN + DNA
1dfm - BsENN (mutant) + DNA

BglII ENN

1es8 - BsENN

MunI ENN

1d02 - ENN (mutant) + DNA - Mycoplasma

Sm ENN

1smn, 1qae, 1ql0, 1g8t – ENN – Serratia marcescens

NaeI ENN

1ev7 - LaENN – Lechevalieria aerocolonigenes
1iaw - LaENN + DNA

NgoMIV ENN

1fiu, 4abt - ENN + DNA – Neisseria gonorrheae

NEI ENN

1tdh - hENN

BsoBI ENN

1dc1 - GsENN + DNA

Bse634I ENN

1knv – GsENN
3v1z – GsENN + DNA
3v20, 3v21 - GsENN (mutant) + DNA

HincII ENN

1kc6, 1tw8, 1xhu, 1xhv, 1tx3, 2gie - HiENN + DNA – Haemophilus influenza
2gig, 2gih, 2gii, 2gij, 3e3y, 3e40, 3e41, 3e42, 3e43, 3e44, 3e45, 3ebc - HiENN (mutant) + DNA
2aud – HiENN

HinDIII ENN

2e52, 3a4k - HiENN + DNA

HinP1I ENN

1ynm – HiENN
2fkc, 2fkh, 2fl3, 2flc - HiENN + DNA

Flap ENN

1mc8 - ENN (mutant) – Pyrococcus horikoshii
3ory– ENN – Desulfurococcus amylolyticus
3q8k, 3q8l, 3q8m - hENN + DNA
1rxv, 1rxw - AfENN + DNA
1ul1 - hENN + PCNA
2izo - SsENN + PCNA – Sulfolobus solfataricus
1a76, 1a77 - MjENN
1b43 – PfENN – Pyrococcus furiosus
1rxz - AfENN + PCNA

DNA mismatch ENN

1odg - EcENN + DNA
1h7u – hENN

Vvn ENN

1ouo – VvENN – Vibrio vulnificus
1oup, 2ivk - VvENN + DNA

BfiI ENN

2c1l – ENN – Bacillus firmus

SfiI ENN

2ezv, 2f03 – ENN + DNA – synthetic

Ecl18kI ENN

2fqz, 2gb7 – ENN + DNA – Enterobacter cloacae

SdaI ENN

2ixs – ENN – Streptomyces diastaticus

PabI ENN

2dvy – ENN – Pyrococcus abyssi

PspGI ENN

3bm3 – ENN – Pyrococcus

NHN ENN

2qgp – ENN – Geobacter metallireducens

UV damage ENN

2j6v, 3bzg, 3c0s – TtENN
3bzj, 3c0l, 3c0q - TtENN (mutant)
4gle - SaENN + DNA

NotI ENN

3bvq - NoENN – Nocardia otitidiscaviarum
3c25 - NoENN + DNA

BpujI ENN

2vla, 3s1s – ENN – Bacillus pumilus

Sau3AI ENN

2reu – ENN C terminal – Staphylococcus aureus

HpyI ENN

3fc3, [[3gox – HpENN99 + DNA – Helicobacter pylori
3or3 – HpENN88 + DNA

PacI ENN

3ldy, 3m7k - PaENN + DNA – Psdeudomonas alcaligenes

Crossover junction ENN

2kpf - ENN – mouse

OkrAI ENN

3odh - ENN + DNA – Oceanobacter kriegii

BspD6I ENN

2ewf - ENN – Bacillus

ThaI ENN

3ndh - TaENN + DNA

Xpf ENN

1z00, 2a1j - hENN C terminal + DNA excision repair protein
2bgw - ApENN + DNA
2bhn - ApENN

Intron-encoded endonuclease

I-PpoI ENN

1a74, 1ipp, 1a73, 1cz0 - PpENN + DNA – Physarum polycephalum
1cyq, 1evw, 2o6m - PpENN (mutant) + DNA
1evx - PpENN

I-CreI ENN

1bp7, 1g9y, 1g9z, 1n3e, 1n3f, 2vbj, 2vbl, 2vbn, 2vbo, 4aqu, 4aqx - CrENN + DNA
1u0c, 1u0d, 1t9i, 1t9j, 2i3p, 2i3q, 4aab, 4aad, 4aae, 4aaf, 4aag - CrENN (mutant) + DNA
2o7m - CrENN

I-CeuI ENN

2ex5 - ENN (mutant) + DNA – Chlamydomonas eugametos

I-DmoI ENN

1b24, 2vs7, 2vs8 - DmENN + DNA – Desulfurococcus mobilis
1mow – DmENN/CrENN + DNA

I-AniI ENN

1p8k, 2qoj, 3uvf - EnENN + DNA – Emericella nidulans
3eh8 - EnENN (mutant) + DNA

I-HmuI ENN

1u3e - ENN + DNA – Bacillus phage spo1

I-TevI ENN

1i3j, 1t2t - T4ENN + DNA
1ln0, 1mk0 – T4ENN (mutant)

I-SceI ENN

1dfa, 1ef0 – yENN – yeast
1gpp – yENN (mutant)
1lws, 1lwt, 1r7m, 3c0w, 3c0x, 3ool - yENN + DNA
3oor - yENN (mutant) + DNA

I-PkoII ENN

2cw7, 2cw8 – ENN – Thermococcus kodakarensis

I-MspI ENN

1sa3, 1yfi, 2fld – ENN + DNA – Moraxella

I-PfuI ENN

1dq3 – PfENN

I-Tsp061I ENN

2dch – ENN – Thermoproteus

I-Vdi141I ENN

3e54 – ENN – Vulcanisaeta distribute

I-MtuI ENN

3ifj, 3igd - MtENN (mutant) – Mycobacterium tuberculosis
2l8l - MtENN

I-MsoI ENN

1m5x, 3fd2 – MoENN + DNA – Monomastix
3ko2 – MoENN (mutant) + DNA

I-GzeMII ENN

4efj – ENN + DNA – Gibberella zeae

I-tRNA ENN

4fz2 – MoENN – Candidatus micrarchaeum