Galactosidase
From Proteopedia
α-Galactosidase (Agal) hydrolyzes the terminal α-galactosyl moiety from glycoproteins and glycolipids. β-Galactosidase (Bgal) hydrolyzes β-galactosides into monosaccharides. Isopropyl-β-d-thiogalactopyranoside (IPTG) induces Bgal activity. Phenylethyl-β-d-thiogalactopyranoside (PETG) is an inhibitor. Galactose, lactose, o-nitrophenyl-β-d-galactoside (ONPG) are substrates. Galactopyranosyl is reaction intermediate. The image at the right correspond to one representative galactosidase, i.e. the crystal structure of galactosidase from Escherichia coli (3e1f). For details on β-galactosidase see Molecular Playground/Beta-galactosidase.
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3D Structures of Galactosidase
Updated on 18-August-2014
α-Galactosidase
3lrk – yAgal 1 (mutant) – yeast
3lrl, 3lrm – yAgal 1 (mutant) + polysaccharide
3lx9, 3lxa, 3lxb, 3lxc, 3tv8 - hAgal A (mutant) – human
3hg2, 3gxn, 1r46, 1r47, 3s5y, 3s5z – hAgal A
For 3hg2 .
1zy9 – TmAgal – Thermotoga maritima
3h53 - hAgal B (mutant)
3a24 – BtAgal – Bacterioides thetaiotamicron
3a5v – Agal I – Mortierella vinacea
3mi6 – Agal – Lactobacillus brevis
1szn – HjAgal – Hypocrea jecorina
1uas – Agal – rice
2xn0, 2xn1 – LaAgal – Lactobacillus acidophilus
4fnp – GsAgal (mutant) – Geobacillus stearothermophilus
4fnq, 4fnr – GsAgal
α-galactosidase binary complex
3h54 - hAgal B (mutant) + polysaccharide
3h55 - hAgal B (mutant) + galactose
3igu - hAgal B (mutant) + intermediate
3hg3, 3hg5 - hAgal A (mutant) + galactose
3gxp - hAgal A + galactose
3hg4 – hAgal A + intermediate
3gxt – hAgal A + imino sugar
1t0o – HjAgal + galactose
2xn2 - LaAgal + galactose
4fns - GsAgal (mutant) + jirimycin derivative
4fnt, 4fnu – GsAgal + galactoside
β-Galactosidase
3og2 – TrBgal – Trichoderma reesei
3muy, 3i3e, 3iap, 3iaq, 3dyp, 1px3, 3sep, 3t0a, 3t2o, 3vd3, 3vd5, 3vda, 4duv – EcBgal (mutant) – Escherichia coli
1hn1, 1dp0, 1f49, 1f4a, 1f4h, 1gho, 1bgl, 1bgm - EcBgal
3dec, 3d3a, 3bga – BtBgal
1yq2 - Bgal (mutant) – Arthrobacter
1uwi, 4eam - SsBgal (mutant) - Sulfolobus solfataricus
1uwq, 4gow – SsBgal
1kwg – TtBgal – Thermus thermophilus
1tg7 – PeBgal – Penicillium
3tts – BcBgal – Bacillus circulans
4ha3, 4ha4 – Bgal – Acidilobus saccharovorans
3w5f – toBgal – tomato
β-Galactosidase binary complex
1xc6 - PeBgal + galactose
3w5g - toBgal + galactose
1kwk – TtBgal + galactose
3ogr – TrBgal + galactose
3ogs - TrBgal + IPTG
3ogv – TrBgal + inhibitor
2ceq, 1uwr, 1uws, 1uwt, 1uwu, 2cer - SsBgal + inhibitor
4ean – SsBgal + indole
3muz, 3i3d, 3dyo, 1px4, 1jyx, 3t08, 3t0b, 3t2p, 3vd4, 3vd9, 3vdc- EcBgal (mutant) + IPTG
3mv0, 3i3b, 3czj, 1jz0, 1jz1, 1jz2, 1jz3, 1jz4, 1jz5, 1jz6 - EcBgal (mutant) + intermediate
3mv1 - EcBgal (mutant) + guanidinium
3e1f, 1jz7 - EcBgal (mutant) + galactose
3t09, 3t0d, 3t2q, 3vd7, 3vdb - EcBgal (mutant) + galactose derivative
1jyn, 1jyy, 1jyz, 1jz8 - EcBgal (mutant) + lactose derivative
1jyn, 1jyw, 1jyv - EcBgal (mutant) + substrate
1jz6 - EcBgal (mutant) + inhibitor
4duw - EcBgal (mutant) + allolactose
4dux - EcBgal (mutant) + ribose
4ckd – EcBgal + antibody
4iug - Bgal + galactose – Aspergillus oryzae
3ob8, 3oba – Bgal – Kluyveromyces lactis
3tty - BcBgal + galactose
3thd – hBgal + jirimycin derivative
3thc - hBgal + galactose
Endo-1,4-β-galactosidase
2xom, 2xon – TmEbgal carbohydrate-binding domain + β-galactotriose
1ups – Ebgal - Clostridium perfringens
2ygl, 2ygm – Ebgal blood group A & B cleaving – Streptococcus pneumoniae
4bg7 – Ebgal – Emericella nidulans
3,6-anhydro-α-L-galactosidase
3p2n – AAgal – Zobellia galactanivorans
4ak5 – BpAAgal – Bacterioides plebeius
4ak6 – BpAAgal (mutant)
4ak7 – BpAAgal (mutant) + polysaccharide
6-phospho-β-D-galactosidase
2pbg, 3pbg, 4pbg, 1pbg – Pgal (mutant) – Lactococcus lacti
2v4v – gal GH59 – Clostridium cellulolyticum
- Created with the participation of Wayne Decatur.
