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Endonuclease

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Template:STRUCTURE 1rva Endonuclease (ENN) cleaves phosphodiester bond within polynucleotide chain. ENN cleaves DNA at a restriction site which is usually a 6-nucleotide palindrome. ENN is restriction site–specific. Various types of ENN differ by their mechanism of action. ENN is used in genetic engineering to make recombinant DNA. ENN requires a restriction site and a cleavage pattern. ENN-I operates on DNA with separate restriction site and cleavage pattern, while ENN-II operates on overlapping restriction site and cleavage pattern. Some ENNs are encoded within introns thus facilitating their mobility. These ENNs or inteins are designated I-ENN. See also

3D structures of endonuclease

Updated on 24-November-2014 {{#tree:id=OrganizedByTopic|openlevels=0|

ENN-I

- 1bix - hENN – human
- 1cfr – ENN – Citrobacter freundii
- 1af5 - CrENN – Chlamydomonas reinhardtii
- 1fzr, 1m0d, 1m0i, 3cae – T7ENN - Enterobacteria phage T7
- 2g7e, 2g7f – VcENN – Vibrio cholera
- 2vnd - VcENN (mutant)
- 2pu3 - VsENN – Vibrio salmonicida

ENN-II

- 1pvi, 1pvu - ENN – Proteus vulgaris
- 2wsh - T4ENN (mutant) - Enterobacteria phage T4
- 3w52 – ENN – Arabidopsis thaliana

ENN-III

- 2abk, 1ako - EcENN – Escherichia coli
- 1orn, 1orp, 1p59 - GsENN + DNA – Geobacillus stearothermophilus

ENN-IV

- 1qtw - EcENN
- 2nqh - EcENN (mutant)
- 1qum – EcENN + DNA
- 2nqj, 2nq9, 4k1g - EcENN (mutant) + DNA
- 1xp3 - ENN – Bacillus anthracis
- 3aal - GkENN – Geobacillus kaustophilus
- 3aam - TtENN – Thermus thermophilus

ENN-V

- 1eni, 1enj, 1enk, 2end - T4ENN
- 1vas - T4ENN + DNA
- 2w35, 2w36, 4b20 - TmENN + DNA – Thermotoga maritima
- 3hd0 - TmENN
- 3ga2 - BsENN (mutant) – Bacillus subtilis
- 3goc - ENN – Streptomyces avermitilis
- 2fcc – T4ENN + DNA

ENN-VII

- 1en7, 1e7d - T4ENN
- 1e7l - T4ENN (mutant)
- 2qnc, 2qnf - T4ENN (mutant) + DNA

ENN-VIII

- 1q39, 1q39 - EcENN
- 1q3b, 1q3c - EcENN (mutant)
- 1tdh – hENN
- 1bix – hENN + DNA
- 1k3x, 2ea0, 1k3w, 1k3x, 2ea0, 2opf, 2oq4 - EcENN + DNA
- 2opf, 2oq4 - EcENN (mutant) + DNA

EcoKI ENN

- 2ar0 – EcENN (mutant)

Ecoo109I ENN

- 1wtd - EcENN
- 1wte – EcENN + DNA

EcoRI ENN

- 1qc9 - EcENN
- 1eri, 1ckq, 1cl8, 1qps – EcENN + DNA
- 1qrh, 1qri, 2oxv - EcENN (mutant) + DNA

EcoRII ENN

- 1na6 – EcENN
- 3hqf, 3hqg - EnENN + DNA

EcoRV ENN

- 1rve, 1az3 – EcENN
- 1az4 - EcENN (mutant)
- 2rve, 4rve, 1rva, 1rvb, 1rvc, 1az0, 1bsu, 1bua, 1bgb, 1rv5, 1b94, 1b95, 1eo3, 1eo4, 1eon, 1eoo, 1eop, 2b0d, 2b0e - EcENN + DNA
- 1bss, 1b96, 1b97, 1stx, 1suz, 1sx5, 1sx8, 2ge5 - EcENN (mutant) + DNA

BamHI ENN

- 1bam – BaENN – Bacillus amyloliquefaciens
- 1bhm, 3bam, 2bam, 1esg - BaENN + DNA

FokI ENN

- 1fok – PoENN + DNA – Planomicrobium okeanokoites
- 2fok – PoENN

PvuII ENN

- 3pvi - PvENN (mutant) + DNA – Proteus vulgaris
- 1k0z, 1h56, 3ksk - PvENN
- 1ni0 - PvENN (mutant)
- 2pvi, 1eyu, 1f0o - PvENN + DNA

tRNA-splicing ENN

- 1a79, 1a76, 1a77 - MjENN – Methanocaldococcus jannaschii
- 2cv8 - ENN – Sulfolobus tokodaii
- 2gw6 – hENN subunit sen15 – NMR
- 2gjw - AfENN + RNA – Archaeoglobus fulgidus
- 1r0v, 1r11 – AfENN
- 2ohc, 2ohe - TaENN – Thermoplasma acidophilum
- 2zyz – ENN – Pyrobaculum aerophilum
- 3iey - ENN + NEQ261 – Nanoarchaeum equitans
- 3ajv - ApENN (mutant) – Aeropyrum pernix
- 3p1y - ApENN /AfENN
- 3p1z - ApENN + protein

VSR ENN

- 1vsr – EcENN
- 1cw0 - EcENN + DNA

BglI ENN

- 1dmu, 1d2i – BsENN + DNA
- 1dfm - BsENN (mutant) + DNA

BglII ENN

- 1es8 - BsENN

MunI ENN

- 1d02 - ENN (mutant) + DNA - Mycoplasma

Sm ENN

- 1smn, 1qae, 1ql0, 1g8t – ENN – Serratia marcescens

NaeI ENN

- 1ev7 - LaENN – Lechevalieria aerocolonigenes
- 1iaw - LaENN + DNA

NgoMIV ENN

- 1fiu, 4abt - ENN + DNA – Neisseria gonorrheae

NEI ENN

- 1tdh - hENN

BsoBI ENN

- 1dc1 - GsENN + DNA

Bse634I ENN

- 1knv – GsENN
- 3v1z – GsENN + DNA
- 3v20, 3v21 - GsENN (mutant) + DNA

HincII ENN

- 1kc6, 1tw8, 1xhu, 1xhv, 1tx3, 2gie - HiENN + DNA – Haemophilus influenza
- 2gig, 2gih, 2gii, 2gij, 3e3y, 3e40, 3e41, 3e42, 3e43, 3e44, 3e45, 3ebc - HiENN (mutant) + DNA
- [2aud]] – HiENN

HinDIII ENN

- 2e52, 3a4k - HiENN + DNA

HinP1I ENN

- 1ynm – HiENN
- 2fkc, 2fkh, 2fl3, 2flc - HiENN + DNA

Flap ENN

- 1mc8 - ENN (mutant) – Pyrococcus horikoshii
- 3ory– ENN – Desulfurococcus amylolyticus
- 3q8k, 3q8l, 3q8m - hENN + DNA
- 1rxv, 1rxw - AfENN + DNA
- 1ul1 - hENN + PCNA
- 2izo - SsENN + PCNA – Sulfolobus solfataricus
- 1a76, 1a77 - MjENN
- 1b43 – PfENN – Pyrococcus furiosus
- 1rxz - AfENN + PCNA

DNA mismatch ENN

- 1odg - EcENN + DNA
- 1h7u – hENN

Vvn ENN

- 1ouo – VvENN – Vibrio vulnificus
- 1oup, 2ivk - VvENN + DNA

BfiI ENN

- 2c1l – ENN – Bacillus firmus

SfiI ENN

- 2ezv, 2f03 – ENN + DNA – synthetic

Ecl18kI ENN

- 2fqz, 2gb7 – ENN + DNA – Enterobacter cloacae

SdaI ENN

- 2ixs – ENN – Streptomyces diastaticus

PabI ENN

- 2dvy – ENN – Pyrococcus abyssi

PspGI ENN

- 3bm3 – ENN – Pyrococcus

NHN ENN

- 2qgp – ENN – Geobacter metallireducens

UV damage ENN

- 2j6v, 3bzg, 3c0s – TtENN
- 3bzj, 3c0l, 3c0q - TtENN (mutant)
- 4gle - SaENN + DNA

NotI ENN

- 3bvq - NoENN – Nocardia otitidiscaviarum
- 3c25 - NoENN + DNA

BpujI ENN

- 2vla, 3s1s – ENN – Bacillus pumilus

Sau3AI ENN

- 2reu – ENN C terminal – Staphylococcus aureus

HpyI ENN

- 3fc3, [[3gox – HpENN99 + DNA – Helicobacter pylori
- 3or3 – HpENN88 + DNA

PacI ENN

- 3ldy, 3m7k - PaENN + DNA – Psdeudomonas alcaligenes

Crossover junction ENN

- 2kpf - ENN – mouse

OkrAI ENN

- 3odh - ENN + DNA – Oceanobacter kriegii

BspD6I ENN

- 2ewf - ENN – Bacillus

ThaI ENN

- 3ndh - TaENN + DNA

Xpf ENN

- 1z00, 2a1j - hENN C terminal + DNA excision repair protein
- 2bgw - ApENN + DNA
- 2bhn - ApENN

Intron-encoded endonuclease

I-PpoI ENN

- 1a74, 1ipp, 1a73, 1cz0 - PpENN + DNA – Physarum polycephalum
- 1cyq, 1evw, 2o6m - PpENN (mutant) + DNA
- 1evx - PpENN

I-CreI ENN

- 1bp7, 1g9y, 1g9z, 1n3e, 1n3f, 2vbj, 2vbl, 2vbn, 2vbo, 4aqu, 4aqx - CrENN + DNA
- 1u0c, 1u0d, 1t9i, 1t9j, 2i3p, 2i3q, 4aab, 4aad, 4aae, 4aaf, 4aag - CrENN (mutant) + DNA
- 2o7m - CrENN

I-CeuI ENN

- 2ex5 - ENN (mutant) + DNA – Chlamydomonas eugametos

I-DmoI ENN

- 1b24, 2vs7, 2vs8 - DmENN + DNA – Desulfurococcus mobilis
- 1mow – DmENN/CrENN + DNA

I-AniI ENN

- 1p8k, 2qoj, 3uvf - EnENN + DNA – Emericella nidulans
- 3eh8 - EnENN (mutant) + DNA

I-HmuI ENN

- 1u3e - ENN + DNA – Bacillus phage spo1

I-TevI ENN

- 1i3j, 1t2t - T4ENN + DNA
- 1ln0, 1mk0 – T4ENN (mutant)

I-SceI ENN

- 1dfa, 1ef0 – yENN – yeast
- 1gpp – yENN (mutant)
- 1lws, 1lwt, 1r7m, 3c0w, 3c0x, 3ool - yENN + DNA
- 3oor - yENN (mutant) + DNA

I-PkoII ENN

- 2cw7, 2cw8 – ENN – Thermococcus kodakarensis

I-MspI ENN

- 1sa3, 1yfi, 2fld – ENN + DNA – Moraxella

I-PfuI ENN

- 1dq3 – PfENN

I-Tsp061I ENN

- 2dch – ENN – Thermoproteus

I-Vdi141I ENN

- 3e54 – ENN – Vulcanisaeta distribute

I-MtuI ENN

- 3ifj, 3igd - MtENN (mutant) – Mycobacterium tuberculosis
- 2l8l - MtENN

I-MsoI ENN

- 1m5x, 3fd2 – MoENN + DNA – Monomastix
- 3ko2 – MoENN (mutant) + DNA

I-GzeMII ENN

- 4efj – ENN + DNA – Gibberella zeae

I-tRNA ENN

- 4fz2 – MoENN – Candidatus micrarchaeum

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Endonuclease

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3D structures of endonuclease

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