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1jfi
From Proteopedia
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| , resolution 2.62Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Gene: | NC2 alpha (DRAP1) (Homo sapiens), NC2 beta (Dr1) (Homo sapiens), tbp (Homo sapiens) | ||||||
| Domains: | COG5150, BUR6, TBP_eukaryotes, SPT15 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, JenaLib, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the NC2-TBP-DNA Ternary Complex
Contents |
Overview
The X-ray structure of a ternary complex of Negative Cofactor 2 (NC2), the TATA box binding protein (TBP), and DNA has been determined at 2.6 A resolution. The N termini of NC2 alpha and beta resemble histones H2A and H2B, respectively, and form a heterodimer that binds to the bent DNA double helix on the underside of the preformed TBP-DNA complex via electrostatic interactions. NC2beta contributes to inhibition of TATA-dependent transcription through interactions of its C-terminal alpha helix with a conserved hydrophobic feature on the upper surface of TBP, which in turn positions the penultimate alpha helix of NC2beta to block recognition of the TBP-DNA complex by transcription factor IIB. Further regulatory implications of the NC2 heterodimer structure are discussed.
Disease
Known diseases associated with this structure: Glioblastoma OMIM:[606417], Huntington disease-like-4 OMIM:[600075], Parkinson disease OMIM:[600075], Spinocerebellar ataxia 17 OMIM:[600075]
About this Structure
1JFI is a Protein complex structure of sequences from Homo sapiens. The following page contains interesting information on the relation of 1JFI with [TATA-Binding Protein]. Full crystallographic information is available from OCA.
Reference
Crystal structure of negative cofactor 2 recognizing the TBP-DNA transcription complex., Kamada K, Shu F, Chen H, Malik S, Stelzer G, Roeder RG, Meisterernst M, Burley SK, Cell. 2001 Jul 13;106(1):71-81. PMID:11461703
Page seeded by OCA on Wed Mar 26 05:54:02 2008
Categories: Homo sapiens | Protein complex | TATA-Binding Protein | Burley, S K. | Chen, H. | Kamada, K. | Malik, S. | Meisterernst, M. | NYSGXRC, New York Structural GenomiX Research Consortium. | Roeder, R G. | Shu, F. | Stelzer, G. | H2a/h2b | Histone | Nc2 | Negative cofactor | New york structural genomix research consortium | Nysgxrc | Protein structure initiative | Psi | Structural genomic | Tata-dna | Tbp | Transcription initiation
