2v3a
From Proteopedia
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CRYSTAL STRUCTURE OF RUBREDOXIN REDUCTASE FROM PSEUDOMONAS AERUGINOSA.
Overview
Crude oil spills represent a major ecological threat because of the, chemical inertness of the constituent n-alkanes. The Gram-negative, bacterium Pseudomonas aeruginosa is one of the few bacterial species able, to metabolize such compounds. Three chromosomal genes, rubB, rubA1, and, rubA2 coding for an NAD(P)H:rubredoxin reductase (RdxR) and two, rubredoxins (Rdxs) are indispensable for this ability. They constitute an, electron transport (ET) pathway that shuttles reducing equivalents from, carbon metabolism to the membrane-bound alkane hydroxylases AlkB1 and, AlkB2. The RdxR-Rdx system also is crucial as part of the oxidative stress, response in archaea or anaerobic bacteria. The redox couple has been, analyzed in detail as a model system for ET processes. We have solved the, structure of RdxR of P. aeruginosa both alone and in complex with Rdx, without the need for cross-linking, and both structures were refined at, 2.40- and 2.45-A resolution, respectively. RdxR consists of two, cofactor-binding domains and a C-terminal domain essential for the, specific recognition of Rdx. Only a small number of direct interactions, govern mutual recognition of RdxR and Rdx, corroborating the transient, nature of the complex. The shortest distance between the redox centers is, observed to be 6.2 A.
About this Structure
2V3A is a Single protein structure of sequence from Pseudomonas aeruginosa with CL, FAD and PEG as ligands. Structure known Active Site: AC1. Full crystallographic information is available from OCA.
Reference
Crystal structure of the electron transfer complex rubredoxin rubredoxin reductase of Pseudomonas aeruginosa., Hagelueken G, Wiehlmann L, Adams TM, Kolmar H, Heinz DW, Tummler B, Schubert WD, Proc Natl Acad Sci U S A. 2007 Jul 24;104(30):12276-81. Epub 2007 Jul 16. PMID:17636129
Page seeded by OCA on Mon Nov 5 13:27:01 2007
