1qzw
From Proteopedia
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| , resolution 4.10Å | |||||||
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| Ligands: | , , , , , | ||||||
| Gene: | 7S RNA (Sulfolobus solfataricus), SRP54 (Sulfolobus solfataricus) | ||||||
| Related: | 1J8M, 1HQ1, 1QZX
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the complete core of archaeal SRP and implications for inter-domain communication
Overview
Targeting of secretory and membrane proteins by the signal recognition particle (SRP) is evolutionarily conserved, and the multidomain protein SRP54 acts as the key player in SRP-mediated protein transport. Binding of a signal peptide to SRP54 at the ribosome is coordinated with GTP binding and subsequent complex formation with the SRP receptor. Because these functions are localized to distinct domains of SRP54, communication between them is essential. We report the crystal structures of SRP54 from the Archaeon Sulfolobus solfataricus with and without its cognate SRP RNA binding site (helix 8) at 4-A resolution. The two structures show the flexibility of the SRP core and the position of SRP54 relative to the RNA. A long linker helix connects the GTPase (G domain) with the signal peptide binding (M) domain, and a hydrophobic contact between the N and M domains relates the signal peptide binding site to the G domain. Hinge regions are identified in the linker between the G and M domains (292-LGMGD) and in the N-terminal part of the M domain, which allow for structural rearrangements within SRP54 upon signal peptide binding at the ribosome.
About this Structure
1QZW is a Single protein structure of sequence from Sulfolobus solfataricus. Full crystallographic information is available from OCA.
Reference
Crystal structure of the complete core of archaeal signal recognition particle and implications for interdomain communication., Rosendal KR, Wild K, Montoya G, Sinning I, Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14701-6. Epub 2003 Dec 1. PMID:14657338
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