4dt3
From Proteopedia
Crystal structure of zinc-charged lysozyme
Structural highlights
Function[LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.[1] Publication Abstract from PubMedZinc is a suitable metal for anomalous dispersion phasing methods in protein crystallography. Structure determination using zinc anomalous scattering has been almost exclusively limited to proteins with intrinsically bound zinc(s). Here, it is reported that multiple zinc ions can easily be charged onto the surface of proteins with no intrinsic zinc-binding site by using zinc-containing solutions. Zn derivatization of protein surfaces appears to be a largely unnoticed but promising method of protein structure determination. Experimental phasing using zinc anomalous scattering.,Cha SS, An YJ, Jeong CS, Kim MK, Lee SG, Lee KH, Oh BH Acta Crystallogr D Biol Crystallogr. 2012 Sep;68(Pt 9):1253-8. doi:, 10.1107/S0907444912024420. Epub 2012 Aug 18. PMID:22948927[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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Categories: Gallus gallus | Lysozyme | An, Y J | Cha, S S | Jeong, C S | Hydrolase
