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spinqualitylabelsall models PDB ID 1sjj Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image
Related:	1HCI, 1AOA, 1DXX, 1QAG, 1cfd
Resources:	FirstGlance, OCA, PDBsum, RCSB
Coordinates:	save as pdb, mmCIF, xml

Cryo-EM Structure of Chicken Gizzard Smooth Muscle alpha-Actinin

Overview

Cryoelectron microscopy was used to obtain a 3-D image at 2.0 nm resolution of 2-D arrays of smooth muscle alpha-actinin. The reconstruction reveals a well-resolved long central domain with 90 degrees of left-handed twist and near 2-fold symmetry. However, the molecular ends which contain the actin binding and calmodulin-like domains, have different structures oriented approximately 90 degrees to each other. Atomic structures for the alpha-actinin domains were built by homology modeling and assembled into an atomic model. Model building suggests that in the 2-D arrays, the two calponin homology domains that comprise the actin-binding domain have a closed conformation at one end and an open conformation at the other end due to domain swapping. The open and closed conformations of the actin-binding domain suggests flexibility that may underlie Ca2+ regulation. The approximately 90 degrees orientation difference at the molecular ends may underlie alpha-actinin's ability to crosslink actin filaments in nearly any orientation.

About this Structure

1SJJ is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

Reference

A 3-D reconstruction of smooth muscle alpha-actinin by CryoEm reveals two different conformations at the actin-binding region., Liu J, Taylor DW, Taylor KA, J Mol Biol. 2004 Apr 16;338(1):115-25. PMID:15050827

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