4tkk

Structural highlights

Function

[HJC_SULSO] A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination; may have some degree of sequence preference in a mobile junction. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are ligated to produce recombinant products. Can cleave all 4 strands 3 bases 3' of the junction center. Cleaves both mobile and immobile junctions. Modifies the structure of the 4-way DNA junction, a model Holliday junction structure. The protein forms multiple complexes with 4-way DNA, suggesting more than 1 homodimer can bind to each junction.^{[1] [2] [3] [4] [5]}

References

1. ↑ Charlebois RL, Singh RK, Chan-Weiher CC, Allard G, Chow C, Confalonieri F, Curtis B, Duguet M, Erauso G, Faguy D, Gaasterland T, Garrett RA, Gordon P, Jeffries AC, Kozera C, Kushwaha N, Lafleur E, Medina N, Peng X, Penny SL, She Q, St Jean A, van der Oost J, Young F, Zivanovic Y, Doolittle WF, Ragan MA, Sensen CW. Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2. Genome. 2000 Feb;43(1):116-36. PMID:10701121
2. ↑ Kvaratskhelia M, White MF. Two Holliday junction resolving enzymes in Sulfolobus solfataricus. J Mol Biol. 2000 Apr 7;297(4):923-32. PMID:10736227 doi:http://dx.doi.org/10.1006/jmbi.2000.3624
3. ↑ Kvaratskhelia M, Wardleworth BN, Norman DG, White MF. A conserved nuclease domain in the archaeal Holliday junction resolving enzyme Hjc. J Biol Chem. 2000 Aug 18;275(33):25540-6. PMID:10940317 doi:http://dx.doi.org/10.1074/jbc.M003420200
4. ↑ Kvaratskhelia M, Wardleworth BN, Bond CS, Fogg JM, Lilley DM, White MF. Holliday junction resolution is modulated by archaeal chromatin components in vitro. J Biol Chem. 2002 Jan 25;277(4):2992-6. Epub 2001 Nov 14. PMID:11709558 doi:http://dx.doi.org/10.1074/jbc.M109496200
5. ↑ Dorazi R, Parker JL, White MF. PCNA activates the Holliday junction endonuclease Hjc. J Mol Biol. 2006 Dec 1;364(3):243-7. Epub 2006 Sep 9. PMID:17011573 doi:http://dx.doi.org/10.1016/j.jmb.2006.09.011