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2es4

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Crystal structure of the Burkholderia glumae lipase-specific foldase in complex with its cognate lipase

Structural highlights

2es4 is a 4 chain structure with sequence from Atcc 33617 and Burkholderia glumae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
NonStd Res:
Related:	1cvl, 1tah, 1qge
Gene:	lifO, lipB (ATCC 33617)
Activity:	Triacylglycerol lipase, with EC number 3.1.1.3
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[LIP_BURGL] Catalyzes the hydrolysis of triglycerides. [LIFO_BURGL] May be involved in the folding of the extracellular lipase during its passage through the periplasm.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Secretion via the type II secretion pathway in Gram-negative bacteria often relies crucially on steric chaperones in the periplasm. Here, we report the crystal structure of the soluble form of a lipase-specific foldase (Lif) from Burkholderia glumae in complex with its cognate lipase. The structure reveals how Lif uses a novel alpha-helical scaffold to embrace lipase, thereby creating an unusually extensive folding platform.

Structure of a membrane-based steric chaperone in complex with its lipase substrate.,Pauwels K, Lustig A, Wyns L, Tommassen J, Savvides SN, Van Gelder P Nat Struct Mol Biol. 2006 Apr;13(4):374-5. Epub 2006 Mar 5. PMID:16518399^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Frenken LG, de Groot A, Tommassen J, Verrips CT. Role of the lipB gene product in the folding of the secreted lipase of Pseudomonas glumae. Mol Microbiol. 1993 Aug;9(3):591-9. PMID:8412705
↑ Pauwels K, Lustig A, Wyns L, Tommassen J, Savvides SN, Van Gelder P. Structure of a membrane-based steric chaperone in complex with its lipase substrate. Nat Struct Mol Biol. 2006 Apr;13(4):374-5. Epub 2006 Mar 5. PMID:16518399 doi:10.1038/nsmb1065

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

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2es4

From Proteopedia

Crystal structure of the Burkholderia glumae lipase-specific foldase in complex with its cognate lipase

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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