2b5v
From Proteopedia
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| , resolution 2.00Å | |||||||
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| Ligands: | |||||||
| Gene: | gdh (Haloferax mediterranei) | ||||||
| Activity: | Glucose 1-dehydrogenase, with EC number 1.1.1.47 | ||||||
| Related: | 2B5W
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of glucose dehydrogenase from Haloferax mediterranei
Overview
The structure of glucose dehydrogenase from the extreme halophile Haloferax mediterranei has been solved at 1.6-A resolution under crystallization conditions which closely mimic the "in vivo" intracellular environment. The decoration of the enzyme's surface with acidic residues is only partially neutralized by bound potassium counterions, which also appear to play a role in substrate binding. The surface shows the expected reduction in hydrophobic character, surprisingly not from changes associated with the loss of exposed hydrophobic residues but rather arising from a loss of lysines consistent with the genome wide-reduction of this residue in extreme halophiles. The structure reveals a highly ordered, multilayered solvation shell that can be seen to be organized into one dominant network covering much of the exposed surface accessible area to an extent not seen in almost any other protein structure solved. This finding is consistent with the requirement of the enzyme to form a protective shell in a dehydrating environment.
About this Structure
2B5V is a Single protein structure of sequence from Haloferax mediterranei. Full crystallographic information is available from OCA.
Reference
Analysis of protein solvent interactions in glucose dehydrogenase from the extreme halophile Haloferax mediterranei., Britton KL, Baker PJ, Fisher M, Ruzheinikov S, Gilmour DJ, Bonete MJ, Ferrer J, Pire C, Esclapez J, Rice DW, Proc Natl Acad Sci U S A. 2006 Mar 28;103(13):4846-51. Epub 2006 Mar 21. PMID:16551747
Page seeded by OCA on Mon Mar 31 02:01:56 2008
