2enx
From Proteopedia
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| , resolution 2.80Å | |||||||
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| Ligands: | , , , | ||||||
| Gene: | ppaC (Streptococcus agalactiae) | ||||||
| Activity: | Inorganic diphosphatase, with EC number 3.6.1.1 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of the family II inorganic pyrophosphatase from Streptococcus agalactiae at 2.8 resolution
Overview
Streptococcus agalactiae, a prokaryote that causes infections in neonates and immunocompromised adults, has a serine/threonine protein kinase (STK) signalling cascade. The structure of one of the targets, a family II inorganic pyrophosphatase, has been solved by molecular replacement and refined at 2.80 A resolution to an R factor of 19.2% (R(free) = 26.7%). The two monomers in the asymmetric unit are related by a noncrystallographic twofold axis, but the biological dimer is formed by a crystallographic twofold. Each monomer contains the pyrophosphate analogue imidodiphosphate (PNP) and three metal ions per active site: two Mn(2+) ions in sites M1 and M2 and an Mg(2+) ion in site M3. The enzyme is in the closed conformation. Like other family II enzymes, the structure consists of two domains (residues 1-191 and 198-311), with the active site located between them. The conformation of Lys298 in the active site is different from those observed previously and it coordinates to the conserved DHH motif in a unique way. The structure suggests that Ser150, Ser194, Ser195 and Ser296 are the most likely targets for the Ser/Thr kinase and phosphatase because they are surface-accessible and either in the active site or in the hinge region between the two domains.
About this Structure
2ENX is a Single protein structure of sequence from Streptococcus agalactiae. Full crystallographic information is available from OCA.
Reference
Structure of the Streptococcus agalactiae family II inorganic pyrophosphatase at 2.80 A resolution., Rantanen MK, Lehtio L, Rajagopal L, Rubens CE, Goldman A, Acta Crystallogr D Biol Crystallogr. 2007 Jun;63(Pt 6):738-43. Epub 2007, May 15. PMID:17505113
Page seeded by OCA on Mon Mar 31 02:51:35 2008
