2f8v

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spinqualitylabelsall models PDB ID 2f8v Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image
, resolution 2.75Å
Ligands:
Gene:	TCAP (Homo sapiens)
Related:	YA5
Resources:	FirstGlance, OCA, PDBsum, RCSB
Coordinates:	save as pdb, mmCIF, xml

Structure of full length telethonin in complex with the N-terminus of titin

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

The Z-disk region defines the lateral boundary of the sarcomere and requires a high level of mechanical strength to provide a stable framework for large filamentous muscle proteins. The level of complexity at this area is reflected by a large number of protein-protein interactions. Recently, we unraveled how the N-terminus of the longest filament component, the giant muscle protein titin, is assembled into an antiparallel (2:1) sandwich complex by the N-terminal titin-binding segment of the Z-disk ligand telethonin/T-cap [Zou, P., Pinotsis, N., Lange, S., Song, Y.H., Popov, A., Mavridis, I., Mayans, O.M., Gautel, M., Wilmanns, M., 2006. Palindromic assembly of the giant muscle protein titin in the sarcomeric Z-disk. Nature 439, 229-233]. In this contribution, we present structural data of a related complex of the titin N-terminus with full-length telethonin. The C-terminus of telethonin remains invisible, suggesting that it does not fold into a defined structure even in the presence of titin. In contrast to the structure with truncated telethonin, a dimer of two titin/telethonin complexes is formed within the crystal environment, potentially indicating the formation of higher oligomers. We further investigated the structure and dynamics of this assembly by small-angle X-ray scattering, circular dichroism, and in vivo complementation data. The data consistently indicate the involvement of the C-terminal part of telethonin into the assembly of two titin/telethonin complexes.

Disease

Known disease associated with this structure: Cardiomyopathy, dilated, 1N OMIM:[604488], Muscular dystrophy, limb-girdle, type 2G OMIM:[604488]

About this Structure

2F8V is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Evidence for a dimeric assembly of two titin/telethonin complexes induced by the telethonin C-terminus., Pinotsis N, Petoukhov M, Lange S, Svergun D, Zou P, Gautel M, Wilmanns M, J Struct Biol. 2006 Aug;155(2):239-50. Epub 2006 Apr 27. PMID:16713295

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