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CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN THE GEF DOMAIN OF THE SALMONELLA TYPHIMURIUM SOPE TOXIN AND HUMAN CDC42

Overview

The bacterial enteropathogen Salmonella typhimurium employs a type III, secretion system to inject bacterial toxins into the host cell cytosol., These toxins transiently activate Rho family GTP-binding protein-dependent, signaling cascades to induce cytoskeletal rearrangements. One of these, translocated Salmonella toxins, SopE, can activate Cdc42 in a Dbl-like, fashion despite its lack of sequence similarity to Dbl-like proteins, the, Rho-specific eukaryotic guanine nucleotide exchange factors. To elucidate, the mechanism of SopE-mediated guanine nucleotide exchange, we have, analyzed the structure of the complex between a catalytic fragment of SopE, and Cdc42. SopE binds to and locks the switch I and switch II regions of, Cdc42 in a conformation that promotes guanine nucleotide release. ... [(full description)]

About this Structure

1GZS is a [Protein complex] structure of sequences from [Homo sapiens] and [Salmonella typhimurium] with SO4 as [ligand]. Structure known Active Site: SA1. Full crystallographic information is available from [OCA].

Reference

Structural basis for the reversible activation of a Rho protein by the bacterial toxin SopE., Buchwald G, Friebel A, Galan JE, Hardt WD, Wittinghofer A, Scheffzek K, EMBO J. 2002 Jul 1;21(13):3286-95. PMID:12093730

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