UBC13 MMS2

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spinqualitylabelsall models Ubc13 with Mms2. Structure determined by Moraes, T.F., Edwards, R.A., McKenna, S., Pastushok, L., Xiao, W., Glover, J.N., Ellison, M.J. Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

==UBC13-MMS2 E2 Complex==

Contents 1 Summary 2 Function 3 Inhibitors 4 Coordinating Enzymes 5 Pathway for DNA Repair 6 Structural Highlights/Important Residues 7 Mechanism 8 References

Summary

Ubc13 is an E2 ubiquitin conjugating enzyme that lacks function without forming a heterodimer with Mms2. Ubc13 is involved in pathways that repair double stranded damage in DNA molecules. When bound to Mms2, Ubc13 will polyubiquinate PCNA, proliferating cell nuclear antigen. Many proteins that Ubc13 interacts with are RING finger proteins such as Traf6.

Function

UBC13 functions as a heterodimer with MMS2, a structurally similar protein to UBC13, but lacking the catalytic Cysteine residue in the active site. The UBC13 E2 complex with MMS2 functions primarily to enhance DNA repair from double stranded breaks.

Inhibitors

Coordinating Enzymes

Pathway for DNA Repair

Structural Highlights/Important Residues

Ubc13 weights 17.6 kDA, and Mms2 is 16.8 kDA. The heterodimer is stable at high stalt concentrations (1 M), suggesting strong interactions between the two. Kd between the Ubc13 and Mms2 is 2 uM.

Mechanism

References