2qow

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spinqualitylabelsall models PDB ID 2qow Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image
, resolution 3.93Å
Ligands:	, , , , ,
Related:	2QOU, 2QOV, 2QOX
Resources:	FirstGlance, OCA, PDBsum, RCSB
Coordinates:	save as pdb, mmCIF, xml

Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. This file contains the 30S subunit of the second 70S ribosome, with spectinomycin bound. The entire crystal structure contains two 70S ribosomes.

Overview

The widely used antibiotic spectinomycin inhibits bacterial protein synthesis by blocking translocation of messenger RNA and transfer RNAs on the ribosome. Here, we show that in crystals of the Escherichia coli 70S ribosome spectinomycin binding traps a distinct swiveling state of the head domain of the small ribosomal subunit. Spectinomycin also alters the rate and completeness of reverse translocation in vitro. These structural and biochemical data indicate that in solution spectinomycin sterically blocks swiveling of the head domain of the small ribosomal subunit and thereby disrupts the translocation cycle.

About this Structure

2QOW is a Protein complex structure of sequences from Escherichia coli and Salmonella typhimurium. Full crystallographic information is available from OCA.

Reference

A steric block in translation caused by the antibiotic spectinomycin., Borovinskaya MA, Shoji S, Holton JM, Fredrick K, Cate JH, ACS Chem Biol. 2007 Aug 17;2(8):545-52. Epub 2007 Aug 10. PMID:17696316

Page seeded by OCA on Mon Mar 31 04:51:35 2008