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This Sandbox is Reserved from January 19, 2016, through August 31, 2016 for use for Proteopedia Team Projects by the class Chemistry 423 Biochemistry for Chemists taught by Lynmarie K Thompson at University of Massachusetts Amherst, USA. This reservation includes Sandbox Reserved 425 through Sandbox Reserved 439.

Contents 1 Format Help 2 OurMolecule 2.1 Introduction 2.2 Additional Features 2.3 Quiz Question 1 2.4 Quiz Question 2 2.5 See Also 2.6 Credits 2.7 References

Format Help

***We may want to consider changing our pdb code to 4c9x rather than 4c9w. The only difference between them is which enantiomer of crizotinib MTH1 is binding to. 4c9w = R and 4c9x = S. The S enantiomer is the one that causes inhibition. --Matt

OurMolecule

Introduction

spinqualitylabelsall models 4c9w, Basic Structure Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

This is a sample scene created with SAT to by Group, and another to make of the protein.

MTH1, also known as 7,8-dihydro-8-oxoguanine triphosphatase is an enzyme in the human body encodet by the NUDT1 gene.

MTH1 is an enzyme also know as 7,8-dihydro-8-oxoguanine triphosphatase that in the human organism is encoded by the NUDT1 gene. It is inhibited by (S)-Crizotinib which induced an increase in DNA single-strand breaks, activated DNA repair in human colon carcinoma cells, and effectively suppressed tumor growth in animal models.

Additional Features

spinqualitylabelsall models pdbcode, Insert caption here Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Quiz Question 1

spinqualitylabelsall models MTH1 protein structure Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

- This scene colors the protein from the N-terminus to the C terminus.

- This scene colors nonpolar residues grey, polar residues red, and ligands bound to the protein green.

Close up of binding interaction - To be made.

Potential Questions:
-Would this protein be expected to embed itself in the cell membrane? Why?
-TBD
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-The questions are expected to have multiple parts, yes?

Quiz Question 2

spinqualitylabelsall models pdbcode, Insert caption here Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

See Also

• MTH1 (7,8-dihydro-8-oxoguanine triphosphatase)

Credits

Introduction - Megi Marina

Overall Structure - Cassandra Martin

Drug Binding Site - Ben Ryter

Additional Features - Colin Hannahan

Quiz Question 1 - Matt Tuttle

Quiz Question 2 - Eric Rice

References

[http://www.nature.com/nature/journal/v508/n7495/pdf/nature13194.pdf Stereospecific targeting of MTH1 by (S)-crizotinib as an anticancer strategy - Huber]