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2h7v
From Proteopedia
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| , resolution 2.600Å | |||||||
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| Ligands: | , | ||||||
| Gene: | MIG5, RAC1 (Homo sapiens), ypkA (Yersinia pseudotuberculosis) | ||||||
| Related: | 2H7O
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Co-crystal structure of YpkA-Rac1
Overview
Yersinia spp. cause gastroenteritis and the plague, representing historically devastating pathogens that are currently an important biodefense and antibiotic resistance concern. A critical virulence determinant is the Yersinia protein kinase A, or YpkA, a multidomain protein that disrupts the eukaryotic actin cytoskeleton. Here we solve the crystal structure of a YpkA-Rac1 complex and find that YpkA possesses a Rac1 binding domain that mimics host guanidine nucleotide dissociation inhibitors (GDIs) of the Rho GTPases. YpkA inhibits nucleotide exchange in Rac1 and RhoA, and mutations that disrupt the YpkA-GTPase interface abolish this activity in vitro and impair in vivo YpkA-induced cytoskeletal disruption. In cell culture experiments, the kinase and the GDI domains of YpkA act synergistically to promote cytoskeletal disruption, and a Y. pseudotuberculosis mutant lacking YpkA GDI activity shows attenuated virulence in a mouse infection assay. We conclude that virulence in Yersinia depends strongly upon mimicry of host GDI proteins by YpkA.
About this Structure
2H7V is a Protein complex structure of sequences from Homo sapiens and Yersinia pseudotuberculosis. Full crystallographic information is available from OCA.
Reference
Yersinia virulence depends on mimicry of host Rho-family nucleotide dissociation inhibitors., Prehna G, Ivanov MI, Bliska JB, Stebbins CE, Cell. 2006 Sep 8;126(5):869-80. PMID:16959567
Page seeded by OCA on Mon Mar 31 03:26:42 2008
Categories: Homo sapiens | Protein complex | Yersinia pseudotuberculosis | Bliska, J B. | Ivanov, M. | Prehna, G. | Stebbins, C E. | Gdi | Gtpase | Rac1 | Yersinia | Yopo | Ypka
