Structural highlights
Function
[MUTH_HAEIN] Sequence-specific endonuclease that cleaves unmethylated GATC sequences. It is involved in DNA mismatch repair (By similarity).
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
MutH initiates mismatch repair by nicking the transiently unmethylated daughter strand 5' to a GATC sequence. Here, we report crystal structures of MutH complexed with hemimethylated and unmethylated GATC substrates. Both structures contain two Ca2+ ions jointly coordinated by a conserved aspartate and the scissile phosphate, as observed in the restriction endonucleases BamHI and BglI. In the hemimethylated complexes, the active site is more compact and DNA cleavage is more efficient. The Lys residue in the conserved DEK motif coordinates the nucleophilic water in conjunction with the phosphate 3' to the scissile bond; the same Lys is also hydrogen bonded with a carbonyl oxygen in the DNA binding module. We propose that this Lys, which is conserved in many restriction endonucleases and is replaced by Glu or Gln in BamHI and BglII, is a sensor for DNA binding and the linchpin that couples base recognition and DNA cleavage.
MutH complexed with hemi- and unmethylated DNAs: coupling base recognition and DNA cleavage.,Lee JY, Chang J, Joseph N, Ghirlando R, Rao DN, Yang W Mol Cell. 2005 Oct 7;20(1):155-66. PMID:16209953[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Lee JY, Chang J, Joseph N, Ghirlando R, Rao DN, Yang W. MutH complexed with hemi- and unmethylated DNAs: coupling base recognition and DNA cleavage. Mol Cell. 2005 Oct 7;20(1):155-66. PMID:16209953 doi:10.1016/j.molcel.2005.08.019
