5e1q

Structural highlights

Function

[AGAL_BACTN] Galactosidase that is able to hydrolyze the alpha-1,6 disaccharide melibiose and the synthetic p-nitrophenyl alpha-galactoside substrate (pNP-Gal), with retention of the anomeric configuration. Does not hydrolyze DNP-Glc or pNP-Glc.^{[1] [2]}

References

1. ↑ Gloster TM, Turkenburg JP, Potts JR, Henrissat B, Davies GJ. Divergence of catalytic mechanism within a glycosidase family provides insight into evolution of carbohydrate metabolism by human gut flora. Chem Biol. 2008 Oct 20;15(10):1058-67. Epub 2008 Oct 9. PMID:18848471 doi:10.1016/j.chembiol.2008.09.005
2. ↑ Okuyama M, Kitamura M, Hondoh H, Kang MS, Mori H, Kimura A, Tanaka I, Yao M. Catalytic mechanism of retaining alpha-galactosidase belonging to glycoside hydrolase family 97. J Mol Biol. 2009 Oct 9;392(5):1232-41. Epub 2009 Jul 30. PMID:19646996 doi:10.1016/j.jmb.2009.07.068