1b8j
From Proteopedia
ALKALINE PHOSPHATASE COMPLEXED WITH VANADATE
Overview
A high resolution crystal structure of Escherichia coli alkaline phosphatase in the presence of vanadate has been refined to 1.9 A resolution. The vanadate ion takes on a trigonal bipyramidal geometry and is covalently bound by the active site serine nucleophile. A coordinated water molecule occupies the axial position opposite the serine nucleophile, whereas the equatorial oxygen atoms of the vanadate ion are stabilized by interactions with both Arg-166 and the zinc metal ions of the active site. This structural complex supports the in-line displacement mechanism of phosphomonoester hydrolysis by alkaline phosphatase and provides a model for the proposed transition state in the enzyme-catalyzed reaction.
About this Structure
1B8J is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
A model of the transition state in the alkaline phosphatase reaction., Holtz KM, Stec B, Kantrowitz ER, J Biol Chem. 1999 Mar 26;274(13):8351-4. PMID:10085061 Page seeded by OCA on Fri May 2 11:12:15 2008
