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Publication Abstract from PubMed

Protein microcrystals magnetically aligned in D2O hydrogels were subjected to neutron diffraction measurements, and reflections were observed for the first time to a resolution of 3.4 A from lysozyme microcrystals ( approximately 10 x 10 x 50 microm). This result demonstrated the possibility that magnetically oriented microcrystals consolidated in D2O gels may provide a promising means to obtain single-crystal neutron diffraction from proteins that do not crystallize at the sizes required for neutron diffraction structure determination. In addition, lysozyme microcrystals aligned in H2O hydrogels allowed structure determination at a resolution of 1.76 A at room temperature by X-ray diffraction. The use of gels has advantages since the microcrystals are measured under hydrated conditions.

Neutron and X-ray single-crystal diffraction from protein microcrystals via magnetically oriented microcrystal arrays in gels.,Tsukui S, Kimura F, Kusaka K, Baba S, Mizuno N, Kimura T Acta Crystallogr D Struct Biol. 2016 Jul 1;72(Pt 7):823-9. doi:, 10.1107/S2059798316007415. Epub 2016 Jun 23. PMID:27377379^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.