1csl
From Proteopedia
CRYSTAL STRUCTURE OF THE RRE HIGH AFFINITY SITE
Overview
The crystal structure of a 28 nt RNA fragment containing the human immunodeficiency virus type 1 (HIV-1) Rev response element high affinity binding site for Rev protein has been solved at 1.6 A resolution. The overall structure of the RRE helix is greatly distorted from A-form geometry by the presence of two purine-purine base-pairs and two single nucleotide bulges. G48 and G71 form a Hoogsteen-type asymmetric base-pair with G71 adopting a syn conformation. The non-canonical regions in the unliganded Rev response element molecule narrow the major groove width with respect to standard A-RNA. The Rev response element structure observed here represents a closed form of the Rev binding site and differs from conformations of the RNA observed previously by solution NMR studies.
About this Structure
Full crystallographic information is available from OCA.
Reference
The structure of the HIV-1 RRE high affinity rev binding site at 1.6 A resolution., Ippolito JA, Steitz TA, J Mol Biol. 2000 Jan 28;295(4):711-7. PMID:10656783 Page seeded by OCA on Fri May 2 13:04:18 2008
