Structural highlights
Function
[PUM2_MOUSE] Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts (By similarity).
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Puf proteins control translation through the interaction of a C-terminal Puf domain with specific sequences present in the 3' untranslated region of messenger RNAs. In Drosophila, binding of the protein Pumilio to mRNA leads to translational repression which is required for anterior/posterior patterning during embryogenesis. The vertebrate Pumilio homologue 2 (Pum2) has been implicated in controlling germ cell development through interactions with the RNA binding proteins deleted in azoospermia (DAZ), DAZ-like (DAZL) and BOULE. We present the 1.6A resolution X-ray crystal structure of the Puf domain from murine Pum2 and demonstrate that this domain is capable of binding with nanomolar affinity to RNA sequences from the hunchback Nanos response element (NRE) and a previously identified Pum2 binding element (PBE).
Structure and RNA binding of the mouse Pumilio-2 Puf domain.,Jenkins HT, Baker-Wilding R, Edwards TA J Struct Biol. 2009 Sep;167(3):271-6. Epub 2009 Jun 18. PMID:19540345[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Jenkins HT, Baker-Wilding R, Edwards TA. Structure and RNA binding of the mouse Pumilio-2 Puf domain. J Struct Biol. 2009 Sep;167(3):271-6. Epub 2009 Jun 18. PMID:19540345 doi:10.1016/j.jsb.2009.06.007
