| Structural highlights
Function
[PDS5_YEAST] Essential for the establishment and maintenance of sister chromatid cohesion at centromere proximal and distal regions during S phase. Also required for chromosomal condensation.[1] [2] [SCC1_YEAST] Cleavable component of the cohesin complex involved in chromosome cohesion during cell cycle. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by ESP1 and dissociates from chromatin, allowing sister chromatids to segregate.[3] [4] [5] [6]
Publication Abstract from PubMed
Sister chromatid cohesion is a fundamental prerequisite to faithful genome segregation. Cohesion is precisely regulated by accessory factors that modulate the stability with which the cohesin complex embraces chromosomes. One of these factors, Pds5, engages cohesin through Scc1 and is both a facilitator of cohesion, and, conversely also mediates the release of cohesin from chromatin. We present here the crystal structure of a complex between budding yeast Pds5 and Scc1, thus elucidating the molecular basis of Pds5 function. Pds5 forms an elongated HEAT repeat that binds to Scc1 via a conserved surface patch. We demonstrate that the integrity of the Pds5-Scc1 interface is indispensable for the recruitment of Pds5 to cohesin, and that its abrogation results in loss of sister chromatid cohesion and cell viability.
Structure of the Pds5-Scc1 Complex and Implications for Cohesin Function.,Muir KW, Kschonsak M, Li Y, Metz J, Haering CH, Panne D Cell Rep. 2016 Feb 24. pii: S2211-1247(16)30094-8. doi:, 10.1016/j.celrep.2016.01.078. PMID:26923589[7]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hartman T, Stead K, Koshland D, Guacci V. Pds5p is an essential chromosomal protein required for both sister chromatid cohesion and condensation in Saccharomyces cerevisiae. J Cell Biol. 2000 Oct 30;151(3):613-26. PMID:11062262
- ↑ Panizza S, Tanaka T, Hochwagen A, Eisenhaber F, Nasmyth K. Pds5 cooperates with cohesin in maintaining sister chromatid cohesion. Curr Biol. 2000 Dec 14-28;10(24):1557-64. PMID:11137006
- ↑ Michaelis C, Ciosk R, Nasmyth K. Cohesins: chromosomal proteins that prevent premature separation of sister chromatids. Cell. 1997 Oct 3;91(1):35-45. PMID:9335333
- ↑ Guacci V, Koshland D, Strunnikov A. A direct link between sister chromatid cohesion and chromosome condensation revealed through the analysis of MCD1 in S. cerevisiae. Cell. 1997 Oct 3;91(1):47-57. PMID:9335334
- ↑ Heo SJ, Tatebayashi K, Kato J, Ikeda H. The RHC21 gene of budding yeast, a homologue of the fission yeast rad21+ gene, is essential for chromosome segregation. Mol Gen Genet. 1998 Jan;257(2):149-56. PMID:9491073
- ↑ Uhlmann F, Lottspeich F, Nasmyth K. Sister-chromatid separation at anaphase onset is promoted by cleavage of the cohesin subunit Scc1. Nature. 1999 Jul 1;400(6739):37-42. PMID:10403247 doi:http://dx.doi.org/10.1038/21831
- ↑ Muir KW, Kschonsak M, Li Y, Metz J, Haering CH, Panne D. Structure of the Pds5-Scc1 Complex and Implications for Cohesin Function. Cell Rep. 2016 Feb 24. pii: S2211-1247(16)30094-8. doi:, 10.1016/j.celrep.2016.01.078. PMID:26923589 doi:http://dx.doi.org/10.1016/j.celrep.2016.01.078
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