Structural highlights
4k8z is a 3 chain structure with sequence from Pyrococcus kodakaraensis (strain kod1). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Ligands: | , , |
| NonStd Res: | |
| Related: | 1wns, 1qht, 4k8x |
| Gene: | pol, pol TK0001, TK0001 (Pyrococcus kodakaraensis (strain KOD1)) |
| Activity: | DNA-directed DNA polymerase, with EC number 2.7.7.7 |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[DPOL_PYRKO] Intein encoded endonucleases are thought to mediate intein mobility by site-specific recombination initiated by endonuclease cleavage at the "homing site" in gene that lack the intein. PI-PkoI recognizes 5'-GATTTAGATCCCTGTACC-3' and PI-PkoII recognizes 5'-CAGCTACTACGGTTAC-3'.
Publication Abstract from PubMed
Replicate it: Structures of KOD and 9 degrees N DNA polymerases, two enzymes that are widely used to replicate DNA with highly modified nucleotides, were solved at high resolution in complex with primer/template duplex. The data elucidate substrate interaction of the two enzymes and pave the way for further optimisation of the enzymes and substrates.
Structures of KOD and 9 degrees N DNA Polymerases Complexed with Primer Template Duplex.,Bergen K, Betz K, Welte W, Diederichs K, Marx A Chembiochem. 2013 Jun 17;14(9):1058-62. doi: 10.1002/cbic.201300175. Epub 2013, Jun 3. PMID:23733496[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bergen K, Betz K, Welte W, Diederichs K, Marx A. Structures of KOD and 9 degrees N DNA Polymerases Complexed with Primer Template Duplex. Chembiochem. 2013 Jun 17;14(9):1058-62. doi: 10.1002/cbic.201300175. Epub 2013, Jun 3. PMID:23733496 doi:10.1002/cbic.201300175
