Structural highlights
2n0s is a 2 chain structure with sequence from Chlre. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Ligands: | , |
| Gene: | hyd1, HYD1, hydA, hydA1, CHLREDRAFT_183963 (CHLRE), PETF (CHLRE) |
| Activity: | Ferredoxin hydrogenase, with EC number 1.12.7.2 |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[FER_CHLRE] Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.
Publication Abstract from PubMed
The transfer of photosynthetic electrons by the ferredoxin PetF to the [FeFe] hydrogenase HydA1 in the microalga Chlamydomonas reinhardtii is a key step in hydrogen production. Electron delivery requires a specific interaction between PetF and HydA1. However, because of the transient nature of the electron-transfer complex, a crystal structure remains elusive. Therefore, we performed protein-protein docking based on new experimental data from a solution NMR spectroscopy investigation of native and gallium-substituted PetF. This provides valuable information about residues crucial for complex formation and electron transfer. The derived complex model might help to pinpoint residue substitution targets for improved hydrogen production.
Structural Insight into the Complex of Ferredoxin and [FeFe] Hydrogenase from Chlamydomonas reinhardtii.,Rumpel S, Siebel JF, Diallo M, Fares C, Reijerse EJ, Lubitz W Chembiochem. 2015 May 25. doi: 10.1002/cbic.201500130. PMID:26010059[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Rumpel S, Siebel JF, Diallo M, Fares C, Reijerse EJ, Lubitz W. Structural Insight into the Complex of Ferredoxin and [FeFe] Hydrogenase from Chlamydomonas reinhardtii. Chembiochem. 2015 May 25. doi: 10.1002/cbic.201500130. PMID:26010059 doi:http://dx.doi.org/10.1002/cbic.201500130
