Structural highlights
Publication Abstract from PubMed
Indigo is an ancient dye uniquely capable of producing the signature tones in blue denim; however, the dyeing process requires chemical steps that are environmentally damaging. We describe a sustainable dyeing strategy that not only circumvents the use of toxic reagents for indigo chemical synthesis but also removes the need for a reducing agent for dye solubilization. This strategy utilizes a glucose moiety as a biochemical protecting group to stabilize the reactive indigo precursor indoxyl to form indican, preventing spontaneous oxidation to crystalline indigo during microbial fermentation. Application of a beta-glucosidase removes the protecting group from indican, resulting in indigo crystal formation in the cotton fibers. We identified the gene coding for the glucosyltransferase PtUGT1 from the indigo plant Polygonum tinctorium and solved the structure of PtUGT1. Heterologous expression of PtUGT1 in Escherichia coli supported high indican conversion, and biosynthesized indican was used to dye cotton swatches and a garment.
Employing a biochemical protecting group for a sustainable indigo dyeing strategy.,Hsu TM, Welner DH, Russ ZN, Cervantes B, Prathuri RL, Adams PD, Dueber JE Nat Chem Biol. 2018 Jan 8. pii: nchembio.2552. doi: 10.1038/nchembio.2552. PMID:29309053[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hsu TM, Welner DH, Russ ZN, Cervantes B, Prathuri RL, Adams PD, Dueber JE. Employing a biochemical protecting group for a sustainable indigo dyeing strategy. Nat Chem Biol. 2018 Jan 8. pii: nchembio.2552. doi: 10.1038/nchembio.2552. PMID:29309053 doi:http://dx.doi.org/10.1038/nchembio.2552
