5xwg

Publication Abstract from PubMed

We have determined a crystal structure of an RNA duplex containing a novel metal-binding motif. The motif is composed of two sheared GoA base pairs, two unpaired A residues and four phosphate groups in close proximity. Four A residues make an A-A-A-A stacking column at the minor groove side and two G bases are highly inclined, thereby forming the pocket-shaped motif at the major groove side. In the present structure, a hydrated Sr2+ ion exists in the pocket and binds to the O6 and N7 atoms of the two G bases and four phosphate groups. According to the previously-reported metal-binding properties to RNA molecules, many of divalent cations, such as Mg2+, Mn2+, Co2+, Zn2+, Ba2+, Pb2+ and Cd2+, may bind to the motif. This metal-binding motif can be used as a modular building block that allows for precise positioning of a single metal ion in functional nucleic acid molecules.

Crystal structure of a novel RNA motif that allows for precise positioning of a single metal ion.,Kanazawa H, Kondo J J Inorg Biochem. 2017 Nov;176:140-143. doi: 10.1016/j.jinorgbio.2017.08.029. Epub, 2017 Sep 21. PMID:28898762^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.