1yhp
From Proteopedia
Solution Structure of Ca2+-free DdCAD-1
Overview
DdCAD-1 is a novel Ca(2+)-dependent cell adhesion molecule that lacks a hydrophobic signal peptide and a transmembrane domain. DdCAD-1 is expressed by the social amoeba Dictyostelium discoideum at the onset of development. It is synthesized as a soluble protein and then transported to the plasma membrane by contractile vacuoles. Here we describe the novel features of the solution structures of Ca(2+)-free and Ca(2+)-bound monomeric DdCAD-1. DdCAD-1 contains two beta-sandwich domains, belonging to the betagamma-crystallin and immunoglobulin fold classes, respectively. Whereas the N-terminal domain has a major role in homophilic binding, the C-terminal domain tethers the protein to the cell membrane. From structural and mutational analyses, we propose a model for the Ca(2+)-bound DdCAD-1 dimer as a basis for understanding DdCAD-1-mediated cell-cell adhesion at the molecular level. Our results provide new insights into Ca(2+)-dependent mechanisms for cell-cell adhesion.
About this Structure
1YHP is a Single protein structure of sequence from Dictyostelium discoideum. Full crystallographic information is available from OCA.
Reference
Solution structures of the adhesion molecule DdCAD-1 reveal new insights into Ca(2+)-dependent cell-cell adhesion., Lin Z, Sriskanthadevan S, Huang H, Siu CH, Yang D, Nat Struct Mol Biol. 2006 Nov;13(11):1016-22. Epub 2006 Oct 22. PMID:17057715 Page seeded by OCA on Sat May 3 16:19:49 2008
Categories: Dictyostelium discoideum | Single protein | Huang, H B. | Lin, Z. | Siu, C H. | Yang, D W. | Calcium | Cell adhesion | Ddcad-1 | Nmr
