The monomeric, fast-acting hormone, mutant insulin B28 Pro --> Asp, has reduced the likelyhood to form dimers and hexamers for therapeutic purposes. Around small phenolic derivatives mutant insulin B28 can create zinc hexamers, this is used as antimicrobial agents in insulin preparation. In the presence of phenol and m-cresol B28 Asp Insulin has been able to form crystals, containing aromatic side chains at the dimer-dimer interfaces and the monomer-monomer interfaces. However, at the monomer-monomer interfaces, there is a higher conformational flexibility in the B chains, caused by the B28 Pro --> Asp mutation. Additionally, this results in the loss of importance in the intermolecular force. It was discovered, that the binding of two m-cresol molecules disordered the beta-strand in a dimer. This indicated the cross link causes a strain on the B chain, which weakens the monomer-monomer interfaces.
Function
Insulin is a hormone produced in the body to regulate the amount of glucose in the blood. The lack of this hormone is what causes diabetes. B28 Asp Insulin, is a modified form of insulin which is known to have many functions. This type of insulin is modified as the proline, is substituted with an aspartic acid residue. The functions of this altered hormone is to prevent the formation of hexamers, which results in a faster-acting insulin. The faster-acting insulin is more beneficial as it can control blood glucose levels in the body in a more improved way.
Disease
Insulin B28 has been found to be involved in the continuous effort to analyze and understand the structure of insulin. The significance of this monomeric, fast-acting hormone is to better treat diabetes. This modified insulin provides a high dose of insulin, which is used to control blood glucose levels in the body.
Relevance
B28 Asp Insulin is known to reduce dimers and hexamers for therapeutic purposes, which mainly include the ability to control blood glucose levels in the body. Additionally, this monomeric insulin is important in investigating the ability to aggregate in the presence of zinc and its effects on different structures in the molecule.
Structural highlights
Alpha Helices,
Beta Strands ,
Loops ,
Turns.
Protein
Ligand
Solvent
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Additionally,
