2hua
From Proteopedia
Solution Structure of CSFV IRES Domain IIa
Overview
Internal ribosome entry site (IRES) RNAs from the hepatitis C virus (HCV) and classical swine fever virus (CSFV) coordinate cap-independent assembly of eukaryotic 48S initiation complexes, consisting of the 40S ribosomal subunit, eukaryotic initiation factor (eIF) 3 and the eIF2/GTP/Met-tRNA(i)(Met) ternary complex. Here, we report that these IRESes also play a functional role during 80S ribosome assembly downstream of 48S complex formation, in promoting eIF5-induced GTP hydrolysis and eIF2/GDP release from the initiation complex. We show that this function is encoded in their independently folded IRES domain II and that it depends both on its characteristic bent conformation and two conserved RNA motifs, an apical hairpin loop and a loop E. Our data suggest a general mode of subunit joining in HCV and HCV-like IRESes.
About this Structure
Full crystallographic information is available from OCA.
Reference
HCV and CSFV IRES domain II mediate eIF2 release during 80S ribosome assembly., Locker N, Easton LE, Lukavsky PJ, EMBO J. 2007 Feb 7;26(3):795-805. Epub 2007 Jan 25. PMID:17255934 Page seeded by OCA on Sun May 4 06:43:17 2008
