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Contents 1 Results 2 Methods 2.1 purification 2.2 crystallization 2.3 in-house data collection 2.4 synchrotron data collection 2.5 data processing 2.6 structure solution 2.7 structure refinement 2.8 making figures 2.9 publishing 2.10 model building 2.11 conferences 3 Discussion 3.1 weekends 3.2 smoking 3.3 secretaries 3.4 IT dept 3.5 occupational hazards 3.6 food supply 3.7 sleeping arrangements 4 Footnotes

Results

spinqualitylabelsall models moving up to macromolecules Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image Contents 1 1980 2 1987 - 2004 3 1988 - 1993 4 1992 5 1994 - 2012 6 1997 7 1998 8 1996 - 2001 9 1999 10 1999 11 2001 - 2011 12 2001 13 2005 14 2017 1980 and refined, 2CTX 1987 - 2004 ... as well as 3RNT[1], 2RNT, 4RNT, 9RNT, 8RNT, 7RNT, 6RNT, 5RNT, 1RN4, 1RGL, 1RGK, 2AAE, 2AAD, 1RGC, 1TRQ, 1TRP, 4BIR, 1CH0, 1Q9E 1988 - 1993 Proteinase K (2PRK) ... as well as 1PEK and 1PTK 1992 Factor for inversion stimulation FIS (1FIA) 1994 - 2012 Tetracycline repressor (2TRT) 1997 Methyltransferase (1AQI) 1998 Arylsulfatase A (1AUK) 1996 - 2001 Photosystem I (1C51) 1999 Cycloamylose 26 1C58 1999 PNP 1QE5 2001 - 2011 Photosystem II (1FE1) 2001 Replicative helicase (1G8Y) 2005 HNA-RNA hybrid (2BJ6) . . . 2017 L-selectin lectin/EGF domains (5VC1)

Methods

The third floor was one big corridor, outlining the steps in structure solution. Cold rooms on one end, crystallization and X-ray labs in the middle (along with the two grown-up offices and the seminar room) and data crunching on the other end.

purification

crystallization

in-house data collection

synchrotron data collection

data processing

structure solution

structure refinement

making figures

publishing

model building

conferences

Discussion

weekends

smoking

secretaries

IT dept

occupational hazards

food supply

sleeping arrangements

Footnotes

1. ↑ Karsten: back then, you could ask for a specific PDB ID, and researchers tried to get one that fit their project. nRNT for RNase T1 is pretty good, but at one point the lab ran out of digits